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Войти в исследования Anticancer research · 2015
Patient-derived orthotopic xenograft (PDOX) nude mouse model of soft-tissue sarcoma more closely mimics the patient behavior in contrast to the subcutaneous ectopic model.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Hiroshima Yukihiko, Zhang Yong, Zhang Nan, Uehara Fuminari, Maawy Ali, Murakami Takashi, Mii Sumiyuki, Yamamoto Mako, Miwa Shinji, Yano Shuya, Momiyama Masashi, Mori Ryutaro, Matsuyama Ryusei, Chishima Takashi, Tanaka Kuniya, Ichikawa Yasushi, Bouvet Michael, Endo Itaru, Hoffman Robert M
Аннотация
AIM: Soft-tissue sarcomas are a group of rare mesenchymal carcinomas that include approximately 50 histological types, and account for 1% of all adult cancer cases. The yearly incidence of soft-tissue sarcomas in the USA is approximately 11,280 cases, with an overall mortality of 3,900 deaths per year.
MATERIALS AND METHODS: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology.
RESULTS: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous high-grade spindle cells of varying sizes, similar to the original patient tumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells.
CONCLUSION: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model.
MATERIALS AND METHODS: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology.
RESULTS: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous high-grade spindle cells of varying sizes, similar to the original patient tumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells.
CONCLUSION: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model.