Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases · 2006
[The microfluorimetric assay (MFA) in in vitro testing the sensitivity of Plasmodium falciparum to antimalarial drugs].
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Huang Fang, Tang Lin-hua, Wang Qin-mei, Hi Yi-chang
Аннотация
OBJECTIVE: To determine the feasibility of the microfluorimetric assay (MFA) in screening antimalarial drugs.
METHODS: The dose-response curves and effective concentration that resulted in a 50% inhibitory of parasitemia (IC50) of chloroquine, artemisinin, artemether and pyronaridine phosphate against Plasmodium falciparum strain FCC1/HN cultured in vitro were tested by microfluorimetric assay (MFA) and compared with those determined by microscopy-based assay.
RESULTS: The IC50 of chloroquine, artemisinin, artemether and pyronaridine phosphate were 18.79, 6.32, 3.67 and 2.00 nmol/L by MFA and the IC50 of which were 19.65, 5.82, 4.38 and 2.83 nmol/L by microscopy-based technique respectively. The results from both techniques were similar or identical (P > 0.05).
CONCLUSION: The microfluorimetric assay (MFA) is sensitive, rapid, easily interpreted and would be useful for testing the sensitivity of Plasmodium falciparum to antimalarials and for screening
METHODS: The dose-response curves and effective concentration that resulted in a 50% inhibitory of parasitemia (IC50) of chloroquine, artemisinin, artemether and pyronaridine phosphate against Plasmodium falciparum strain FCC1/HN cultured in vitro were tested by microfluorimetric assay (MFA) and compared with those determined by microscopy-based assay.
RESULTS: The IC50 of chloroquine, artemisinin, artemether and pyronaridine phosphate were 18.79, 6.32, 3.67 and 2.00 nmol/L by MFA and the IC50 of which were 19.65, 5.82, 4.38 and 2.83 nmol/L by microscopy-based technique respectively. The results from both techniques were similar or identical (P > 0.05).
CONCLUSION: The microfluorimetric assay (MFA) is sensitive, rapid, easily interpreted and would be useful for testing the sensitivity of Plasmodium falciparum to antimalarials and for screening