Molecular and cellular endocrinology · 2025
The N-terminal region of HASPIN regulates phosphorylation of AURKA and meiotic progression in spermatocytes.
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Li Haojie, Xu Yaoting, Jiang Xinyi, Ren Jie, Wang Yulian, Zhang Xiangzheng, Gao Mengmeng, Zhang Longsheng, Wang Yue, Li Zongze, Wang Suwei, Wang Tianye, Wang Mengyi, Situ Chenghao, Guo Xuejiang, Zhu Hui
Аннотация
Protein phosphorylation is an important post-translational modification that plays a critical regulatory role in meiosis. HASPIN, a kinase highly conserved from yeast to mammals, is required for male fertility. In this study, we found that the intrinsically disordered N-terminal domain of HASPIN is also required for this function. Mice with deletion of N-terminal amino acids (aa) 1-243 of HASPIN exhibited reduced testicular size, sperm count, and fertility. Using immunoprecipitation-mass spectrometry and phosphoproteomics analysis, we found that HASPIN could interact with AURKA and regulate its phosphorylation at T279 via its N-terminus. Taken together, our results suggest that the N-terminus of HASPIN regulates AURKA kinase activity to affect male fertility.