Clinical oral investigations · 2025
The potential of resolvin D2 loaded hydroxyapatite/mineral trioxide aggregate electrospun membranes to downregulate Interleukin 1 beta and tumor necrosis factor alpha in human dental pulp stem cells and macrophages co-cultures.
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Sheela Soumya, AlGhalban Fatma Mousa, Khalil Khalil Abdelrazek, Gopinath Vellore Kannan
Аннотация
OBJECTIVE: To develop electrospun polycaprolactone (PCL) membranes incorporating Hydroxyapatite/Mineral Trioxide Aggregate (HAp/MTA) loaded with Resolvin D2 (RvD2) and evaluate its anti-inflammatory potential.
METHOD: Electrospun membranes were fabricated by blending PCL with 10 wt.% HAp/10 wt.% MTA loaded with or without 0.1% RvD2 and were characterized using Field Emission Scanning Electron Microscopy (FE-SEM), energy-dispersive X-ray (EDX) spectroscopy, Raman spectroscopy, contact angle measurements, and RvD2 release.
RESULTS: The field emission scanning microscopy (FE-SEM) showed uniform nanofibers with an average fiber diameter ranging from 300 to 600 nm. Energy-dispersive X-ray (EDX) analysis and Raman spectroscopy confirmed the presence of HAp and MTA in the PCL membranes. The drug release profile displayed a rapid release of RvD2 initially, followed by a sustained release. XTT assay demonstrated the cytocompatibility of the membranes towards the THP-1-derived M1 macrophages and human dental pulp stem cells (hDPSCs) co-cultures activated with LPS. The FE-SEM studies proved the favorable cell adhesion behaviour although the membranes being hydrophobic. Cytokine levels in the culture supernatants retrieved from the co-culture treated membranes showed that, of all the membranes, RvD2 incorporated PCLHAp/MTA membranes demonstrated reduced IL-1β and TNF-α production.
CONCLUSIONS: RvD2 incorporated PCLHAp/MTA membranes demonstrated reduced IL-1β and TNF-α production asserting its anti-inflammotory potential.
CLINICAL SIGNIFICANCE: This research highlights the immunomodulatory effect of RvD2-loaded PCLHAp/MTA electrospun membranes in reducing inflammation of the dental pulp. This novel method has the potential to transform the treatment of dental pulp inflammation resulting from caries or injury.
METHOD: Electrospun membranes were fabricated by blending PCL with 10 wt.% HAp/10 wt.% MTA loaded with or without 0.1% RvD2 and were characterized using Field Emission Scanning Electron Microscopy (FE-SEM), energy-dispersive X-ray (EDX) spectroscopy, Raman spectroscopy, contact angle measurements, and RvD2 release.
RESULTS: The field emission scanning microscopy (FE-SEM) showed uniform nanofibers with an average fiber diameter ranging from 300 to 600 nm. Energy-dispersive X-ray (EDX) analysis and Raman spectroscopy confirmed the presence of HAp and MTA in the PCL membranes. The drug release profile displayed a rapid release of RvD2 initially, followed by a sustained release. XTT assay demonstrated the cytocompatibility of the membranes towards the THP-1-derived M1 macrophages and human dental pulp stem cells (hDPSCs) co-cultures activated with LPS. The FE-SEM studies proved the favorable cell adhesion behaviour although the membranes being hydrophobic. Cytokine levels in the culture supernatants retrieved from the co-culture treated membranes showed that, of all the membranes, RvD2 incorporated PCLHAp/MTA membranes demonstrated reduced IL-1β and TNF-α production.
CONCLUSIONS: RvD2 incorporated PCLHAp/MTA membranes demonstrated reduced IL-1β and TNF-α production asserting its anti-inflammotory potential.
CLINICAL SIGNIFICANCE: This research highlights the immunomodulatory effect of RvD2-loaded PCLHAp/MTA electrospun membranes in reducing inflammation of the dental pulp. This novel method has the potential to transform the treatment of dental pulp inflammation resulting from caries or injury.
Предметные рубрики MeSH
HumansDental PulpStem CellsDrug CombinationsMacrophagesInterleukin-1betaSilicatesCalcium CompoundsOxidesAluminum CompoundsTumor Necrosis Factor-alphaCoculture TechniquesMicroscopy, Electron, ScanningPolyestersDown-RegulationDocosahexaenoic AcidsSpectrum Analysis, RamanSpectrometry, X-Ray EmissionDurapatiteMembranes, ArtificialCells, CulturedDrug Liberation