BMC urology · 2012
Identification of prostaglandin receptors in human ureters.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Oll Matthias, Baumann Claudia, Behbahani Turang E, von Ruecker Alexander, Müller Stefan C, Ellinger Jörg
Аннотация
BACKGROUND: Prostaglandins play an important role in ureteral obstruction, but the detailed expression profiles of the prostaglandin receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR) remain unknown in the different parts of the human ureter.
METHODS: The expression pattern of PTGER1, PTGER2, PTGER3, PTGER4 and PTGFR was determined in human distal, mid and proximal ureter and renal pelvis samples using immunohistochemistry (protein levels) and quantitative real-time PCR (mRNA).
RESULTS: PTGER1 was highly expressed in most samples irrespective of the ureteral localization; however, urothelial cells had higher levels of PTGER1 than smooth muscle cells. PTGFR was also moderately to strongly expressed in urothelial and smooth muscle cells. In comparison, PTGER2-4 expression was mostly unexpressed or weakly expressed in urothelial and smooth cells in all regions.
CONCLUSIONS: Our data indicate high levels of PTGER1 in ureters.
METHODS: The expression pattern of PTGER1, PTGER2, PTGER3, PTGER4 and PTGFR was determined in human distal, mid and proximal ureter and renal pelvis samples using immunohistochemistry (protein levels) and quantitative real-time PCR (mRNA).
RESULTS: PTGER1 was highly expressed in most samples irrespective of the ureteral localization; however, urothelial cells had higher levels of PTGER1 than smooth muscle cells. PTGFR was also moderately to strongly expressed in urothelial and smooth muscle cells. In comparison, PTGER2-4 expression was mostly unexpressed or weakly expressed in urothelial and smooth cells in all regions.
CONCLUSIONS: Our data indicate high levels of PTGER1 in ureters.
Предметные рубрики MeSH
Gene Expression ProfilingHumansImmunohistochemistryKidney PelvisMyocytes, Smooth MuscleRNA, MessengerReal-Time Polymerase Chain ReactionReceptors, ProstaglandinReceptors, Prostaglandin EReceptors, Prostaglandin E, EP1 SubtypeReceptors, Prostaglandin E, EP2 SubtypeReceptors, Prostaglandin E, EP3 SubtypeReceptors, Prostaglandin E, EP4 SubtypeUreterUreteral ObstructionUrothelium