Authentication of Panax notoginseng with high-efficiency Proofman-LMTIA technology.

Panax notoginseng, a cornerstone of traditional Chinese medicine, is frequently subject to adulteration in commercial markets, compromising its therapeutic efficacy and safety. This study introduces a novel application of Proofman-LMTIA technology to authenticate P. notoginseng with high precision and efficiency. By targeting unique sequence variations in the ITS2 rDNA region, we developed species-specific primers and probe to distinguish P. notoginseng from common adulterants. The method achieves a detection sensitivity of 10 pg/µL and identifies adulteration at levels as low as 1 % (v/v), validated across diverse commercial products, including powders and capsules. With a detection time of under 30 min and no reliance on specialized equipment, this approach offers a streamlined, cost-efficient solution for quality assurance in the herbal industry. Our results demonstrate 100 % accuracy in market sample testing, addressing critical challenges in P. notoginseng authentication and supporting regulatory compliance.