Journal of ginseng research · 2026
Ginsenoside Rb2 modulates the skin barrier by targeting Src to regulate PI3K/Akt signaling in HaCaT cells.
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Hu Haiyue, Yuan Linli, Feng Jiale, Zhang Ying, Zhang Zuo, Zhang Jinglin, Jin Lei, Wu Haifeng, You Long, Wang Xinfeng, Zhang Ji, Hu Weicheng
Аннотация
BACKGROUND: Ginsenoside Rb2 (G-Rb2) is a protopanaxadiol-type saponin, which is primarily enriched in the stems, leaves, and berries. G-Rb2 exhibits multiple biological effects, mainly including anti-diabetic, anti-obesity, cardiovascular protection, and anti-virus activities. However, the mechanism by which G-Rb2 regulates skin health has not been clearly elucidated.
METHODS: In this study, RNA sequencing (RNA-seq) was employed to analyze gene expression profiles in HaCaT cells treated with G-Rb2. Based on the bioinformatic results, HaCaT cells were treated with different concentrations of G-Rb2, and several in vitro cellular experiments were conducted to investigate the underlying mechanisms by which G-Rb2 regulates skin health. To further clarify the interaction between G-Rb2 and Src, molecular modeling approaches including density functional theory (DFT) calculations, molecular docking, and molecular dynamics (MD) simulations were performed.
RESULTS: The results demonstrated that G-Rb2 exerted a pronounced skin-protective effect by activating Src and regulating skin health via the downstream PI3K/Akt signaling pathway. DFT-derived electrostatic potential and frontier orbital analyses, molecular docking, and 100-ns MD simulations collectively supported a stable binding mode of G-Rb2 to Src.
CONCLUSION: These findings highlighted the potential of G-Rb2 as a natural functional compound for supporting skin health and preventing skin-associated disorders.
METHODS: In this study, RNA sequencing (RNA-seq) was employed to analyze gene expression profiles in HaCaT cells treated with G-Rb2. Based on the bioinformatic results, HaCaT cells were treated with different concentrations of G-Rb2, and several in vitro cellular experiments were conducted to investigate the underlying mechanisms by which G-Rb2 regulates skin health. To further clarify the interaction between G-Rb2 and Src, molecular modeling approaches including density functional theory (DFT) calculations, molecular docking, and molecular dynamics (MD) simulations were performed.
RESULTS: The results demonstrated that G-Rb2 exerted a pronounced skin-protective effect by activating Src and regulating skin health via the downstream PI3K/Akt signaling pathway. DFT-derived electrostatic potential and frontier orbital analyses, molecular docking, and 100-ns MD simulations collectively supported a stable binding mode of G-Rb2 to Src.
CONCLUSION: These findings highlighted the potential of G-Rb2 as a natural functional compound for supporting skin health and preventing skin-associated disorders.