A major step towards a practical and meaningful gluten analysis.

Coeliac disease is triggered by the cereal protein gluten. Elimination of gluten is the effective therapy. To increase food safety for coeliac patients, gluten has been included in food regulations introducing obligatory labelling. These regulations depend upon effective analytical control. A combined system using cocktail extraction and the R5 enzyme-linked immunosorbent assay has now been described for gluten analysis, which shows good performance. Every effort should be made to keep the diet of coeliac patients as gluten-free as possible.