Bio-medical materials and engineering · 2025
Experimental study of extracellular vesicle-small intestine submucosa novel biological composite for urethral stricture repair and reconstruction.
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Wang Dan, Zhu Xiaojun, Siqin Buhe, Ren Chao, Chang Ming, Bai Ligang
Аннотация
BACKGROUND: Urethral stricture (US) is a common condition that considerably affects patients' quality of life.
OBJECTIVES: This study aimed to explore the adoption value of the extracellular vesicle (EV)- small intestinal submucosa (SIS) complex in the repair of USs.
METHODS: EVs were extracted from healthy male New Zealand white rabbits, and SIS was prepared using peracetic acid (PAA) oxidation and decellularization. The morphology and particle size of the prepared EV-SIS complex were evaluated using electron microscopy and qNano nanoparticle analyzer, and the labeled proteins of EVs were detected using Western blot method. EV-SIS the complex was implanted in a rabbit model of US, and urodynamic parameters were assessed.
RESULTS: The EV-SIS complex displayed a full morphology, intact membrane structure, and uniform particle size. The protein concentration of EVs in the complex was approximately 0.351 µg/µL, with a yield of approximately 1.86 µg/106 cells. The complex exhibited remarkable repair effects in the rabbit model of US, with bladder capacity, maximal urethral pressure, and minimal urethral pressure all markedly superior to those in the US group (P < 0.05).
CONCLUSION: The EV-SIS complex demonstrates potential clinical value in the repair of USs, improving urodynamic parameters, and offering a promising therapeutic option for patients with US.
OBJECTIVES: This study aimed to explore the adoption value of the extracellular vesicle (EV)- small intestinal submucosa (SIS) complex in the repair of USs.
METHODS: EVs were extracted from healthy male New Zealand white rabbits, and SIS was prepared using peracetic acid (PAA) oxidation and decellularization. The morphology and particle size of the prepared EV-SIS complex were evaluated using electron microscopy and qNano nanoparticle analyzer, and the labeled proteins of EVs were detected using Western blot method. EV-SIS the complex was implanted in a rabbit model of US, and urodynamic parameters were assessed.
RESULTS: The EV-SIS complex displayed a full morphology, intact membrane structure, and uniform particle size. The protein concentration of EVs in the complex was approximately 0.351 µg/µL, with a yield of approximately 1.86 µg/106 cells. The complex exhibited remarkable repair effects in the rabbit model of US, with bladder capacity, maximal urethral pressure, and minimal urethral pressure all markedly superior to those in the US group (P < 0.05).
CONCLUSION: The EV-SIS complex demonstrates potential clinical value in the repair of USs, improving urodynamic parameters, and offering a promising therapeutic option for patients with US.