Molecular biology reports · 2026
Growth factor betacellulin can stimulate human granulosa cell functions and promote gonadotropin actions.
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Loncová Barbora, Fabová Zuzana, Mlynček Miloš, Sirotkin Alexander V
Аннотация
BACKGROUND: Betacellulin (BTC), an EGF-family growth factor expressed in granulosa cells, is implicated in ovarian regulation; however, its interactions with gonadotropins in humans remain unclear.
OBJECTIVE: To determine how BTC alone or combined with follicle-stimulating hormone (FSH) or luteinizing hormone (LH) affects human granulosa cell viability, proliferation, apoptosis, and progesterone secretion.
METHODS: Human granulosa cells were treated with BTC (1-100 ng/ml) ± FSH or LH (100 ng/ml). Viability was measured by Trypan blue exclusion; proliferation and apoptosis by immunocytochemistry; and progesterone by ELISA.
RESULTS: BTC dose-dependently increased viability, proliferation (cyclin B1, PCNA), and progesterone secretion, while reducing apoptosis (bax, caspase-3). Both FSH and LH enhanced all BTC effects, further increasing proliferation, viability, progesterone output, and anti-apoptotic activity.
CONCLUSION: BTC may regulate human granulosa cell function, acting in concert with gonadotropins to modulate viability, proliferation, apoptosis, and steroidogenesis, highlighting its role as a paracrine mediator in ovarian physiology.
OBJECTIVE: To determine how BTC alone or combined with follicle-stimulating hormone (FSH) or luteinizing hormone (LH) affects human granulosa cell viability, proliferation, apoptosis, and progesterone secretion.
METHODS: Human granulosa cells were treated with BTC (1-100 ng/ml) ± FSH or LH (100 ng/ml). Viability was measured by Trypan blue exclusion; proliferation and apoptosis by immunocytochemistry; and progesterone by ELISA.
RESULTS: BTC dose-dependently increased viability, proliferation (cyclin B1, PCNA), and progesterone secretion, while reducing apoptosis (bax, caspase-3). Both FSH and LH enhanced all BTC effects, further increasing proliferation, viability, progesterone output, and anti-apoptotic activity.
CONCLUSION: BTC may regulate human granulosa cell function, acting in concert with gonadotropins to modulate viability, proliferation, apoptosis, and steroidogenesis, highlighting its role as a paracrine mediator in ovarian physiology.