A novel fluorescent probe for real-time in vivo imaging of H2O2 level in polycystic ovary syndrome.

This work presents LH, a hemicyanine-based activatable fluorescent probe for the sensitive detection of hydrogen peroxide (H2O2). LH undergoes H2O2-triggered cleavage of the boronate recognition unit, resulting in a turn-on near-infrared (NIR) fluorescence response. The probe exhibited favorable sensitivity, with a limit of detection (LOD) of 0.04 μM, as well as good selectivity and biocompatibility under the tested conditions. Cellular imaging experiments demonstrated that LH could monitor intracellular H2O2 changes in ovarian-derived cells. Furthermore, LH was applied to PCOS-model mice and serum samples, where enhanced fluorescence signals were observed compared with healthy controls, suggesting increased H2O2-associated oxidative changes in the PCOS model. These results indicate that LH may serve as a useful molecular imaging tool for studying oxidative stress-related changes in PCOS-model mice.