Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control · 2013
[Preliminary studies on protective immunity induced by recombinant protein of Schistosoma japonicum P14 gene and its mechanism against schistosome infection in mice].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Tang Xiao-niu, Ji Feng, Jiang Yu-xin, Zhan Xiao-dong, Li Chao-pin
Аннотация
OBJECTIVE: To explore the immune protective effect of the basic calponin-like protein (SjP14) in mice response to the infection of Schistosoma japonicum.
METHODS: The recombinant plasmid pET28a(+)-SjP14 was built, and transferred into E. coli BL21 cell line. After induced, by isopropyl-beta-D-thiogalactoside (IPTG), the target protein was purified, and then analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting, respectively. Thirty female BALB/c mice (six-week age) were randomly divided into three groups (n = 10 for each), including A group (rSjP14 experimental), B group (adjuvant control), and C group (saline control). The mice in the A group were subcutaneously injected with 100 microg rSjP14 vaccine for three times at 2-week interval. After the final immunization, the mice were infected by cercariae of S. japonicum. The B or C groups experienced subcutaneous injections of adjuvant or saline, respectively, except for cercaria infection. The levels of sera IgG, IL-4 and IFN-gamma were detected by ELISA before immunizing, 6 weeks after immunizing, and 6 weeks after the infection, respectively. The reduction rates of worms or eggs were also calculated at the 6th week after the infection.
RESULTS: The SjP14 protein was about 38 kDa and was recognized successfully by polyclonal antibody of SjP14. The level of serum IgG in the A group at the 6th week after the infection of cercariae was significant increased [ (25.52 +/- 1.91) microg/ml] compared with that in the B [ (18.65 +/- 3.16) microg/ml] and C [(22.44 +/- 2.83) mcirog/ml] groups (P < 0.05); the level of serum IFN-gamma [(171.30 +/- 70.12) ng/L] was also increased, compared with that in the B [(136.89 +/- 37.62) ng/L] and C [(153.64 +/- 43.44) ng/L] groups (P < 0.05). However, the level of serum IL-4 [(112.05 +/- 15.02) ng/L] in A group was significant decreased at the 6th week after the infection, compared with that in the C group [(102.82 +/- 27.46) ng/L] (P < 0.05). The reduction rates of worms and eggs in the A group were 29.2% and 41.3%, respectively, after the infection, which had statistically significant differences compared with those of the C group (P < 0.05).
CONCLUSION: SjP14 has a protective immunity against S. japonicum infection in mice.
METHODS: The recombinant plasmid pET28a(+)-SjP14 was built, and transferred into E. coli BL21 cell line. After induced, by isopropyl-beta-D-thiogalactoside (IPTG), the target protein was purified, and then analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting, respectively. Thirty female BALB/c mice (six-week age) were randomly divided into three groups (n = 10 for each), including A group (rSjP14 experimental), B group (adjuvant control), and C group (saline control). The mice in the A group were subcutaneously injected with 100 microg rSjP14 vaccine for three times at 2-week interval. After the final immunization, the mice were infected by cercariae of S. japonicum. The B or C groups experienced subcutaneous injections of adjuvant or saline, respectively, except for cercaria infection. The levels of sera IgG, IL-4 and IFN-gamma were detected by ELISA before immunizing, 6 weeks after immunizing, and 6 weeks after the infection, respectively. The reduction rates of worms or eggs were also calculated at the 6th week after the infection.
RESULTS: The SjP14 protein was about 38 kDa and was recognized successfully by polyclonal antibody of SjP14. The level of serum IgG in the A group at the 6th week after the infection of cercariae was significant increased [ (25.52 +/- 1.91) microg/ml] compared with that in the B [ (18.65 +/- 3.16) microg/ml] and C [(22.44 +/- 2.83) mcirog/ml] groups (P < 0.05); the level of serum IFN-gamma [(171.30 +/- 70.12) ng/L] was also increased, compared with that in the B [(136.89 +/- 37.62) ng/L] and C [(153.64 +/- 43.44) ng/L] groups (P < 0.05). However, the level of serum IL-4 [(112.05 +/- 15.02) ng/L] in A group was significant decreased at the 6th week after the infection, compared with that in the C group [(102.82 +/- 27.46) ng/L] (P < 0.05). The reduction rates of worms and eggs in the A group were 29.2% and 41.3%, respectively, after the infection, which had statistically significant differences compared with those of the C group (P < 0.05).
CONCLUSION: SjP14 has a protective immunity against S. japonicum infection in mice.