Engineering robust Saccharomyces cerevisiae cell factory for improving fatty alcohol biosynthesis.

Engineering yeast cell factories is a feasible approach to produce value chemicals from renewable feedstocks. However, during the production process, reprogramming of the internal metabolic pathways of yeast cells and environmental stress always compromises its production performance. Here, we engineered the robust Saccharomyces cerevisiae to enhance the production of fatty alcohols by downregulating the expression of target of rapamycin gene TOR1 and deleting histone deacetylase gene HDA1 in S. cerevisiae. The enhanced cellular robustness resulted in the extended chronological lifespan (CLS) through metabolic balance and stress response regulation, thus increasing the production of fatty alcohols by up to 56 %. This strategy may be used as a general strategy for building effective microbial cell factories.