Turkiye parazitolojii dergisi · 2013
[Cloning of Blastocystis sp subtype 3 small-subunit ribosomal DNA].
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Şakalar Çağrı, Uyar Yunus, Yürürdurmaz Mehmet Ali, Tokar Sadık, Yeşilkaya Huzeyfe, Gürbüz Esra, Kuk Salih, Yazar Süleyman
Аннотация
OBJECTIVE: Different sub-types or genetic variations of Blastocystis sp. are thought to play a role in the differential symptoms caused by the parasite or asymptomatic cases. In this study, it was aimed to clone a fragment of SSUrDNA gene of Blastocystis from a patient in order to define its phylogenetic subtype.
METHODS: In this study, DNA isolation from the stool of a Blastocystis infected patient was performed. Blastocystis specific primers were used to amplify a SSUrDNA genomic fragment. The amplified DNA fragment was cloned into a plasmid and sequenced using plasmid specific primers. The obtained DNA sequence was analyzed using BLAST and a phylogenetic tree was constructed using the software MEGA.
RESULTS: It was found that the Blastocystis isolate in our study is subtype 3.
CONCLUSION: Cloning and sequencing of the target genomic region is suggested for phylogenetic analysis studies.
METHODS: In this study, DNA isolation from the stool of a Blastocystis infected patient was performed. Blastocystis specific primers were used to amplify a SSUrDNA genomic fragment. The amplified DNA fragment was cloned into a plasmid and sequenced using plasmid specific primers. The obtained DNA sequence was analyzed using BLAST and a phylogenetic tree was constructed using the software MEGA.
RESULTS: It was found that the Blastocystis isolate in our study is subtype 3.
CONCLUSION: Cloning and sequencing of the target genomic region is suggested for phylogenetic analysis studies.