Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2004
[A novel microbeads-based chemiluminescence immunoassay for detecting human thyrotropin].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Wang Yong-ning, Li Rong-xiu, Li Cheng-wu, Gao Yun-hua, Guo Xiao-ying, Gu Zhong-ze
Аннотация
AIM: To establish a low cost and sensitive microbeads-based chemiluminescence immunoassay (CLEIA) for detecting human thyrotropin.
METHODS: Thyrotropin in sera was captured by an alkaline phosphase-labeled mAb against TSH beta subunit and a FITC-labeled mAb against TSH alpha subunit. Captured thyrotropin was then isolated with immuno-magnetic beads conjugated with anti-FITC antibody and then quantified by chemiluminescence using adamanatane amine as luminescent substrate.
RESULTS: The sensitivity of this assay is 0.004 mIU/L. The intra-assay CV and the inter-assay CV of the assay were 7.45% and 10.45%, respectively. The recovery rate was 91.4% -102.4%. The examination result of the assay correlates well with that of ACS-180 system.
CONCLUSION: The CLELA for detecting TSH is low-cost, sensitive, specific and stable, and therefore may have a promising prospect in clinical application.
METHODS: Thyrotropin in sera was captured by an alkaline phosphase-labeled mAb against TSH beta subunit and a FITC-labeled mAb against TSH alpha subunit. Captured thyrotropin was then isolated with immuno-magnetic beads conjugated with anti-FITC antibody and then quantified by chemiluminescence using adamanatane amine as luminescent substrate.
RESULTS: The sensitivity of this assay is 0.004 mIU/L. The intra-assay CV and the inter-assay CV of the assay were 7.45% and 10.45%, respectively. The recovery rate was 91.4% -102.4%. The examination result of the assay correlates well with that of ACS-180 system.
CONCLUSION: The CLELA for detecting TSH is low-cost, sensitive, specific and stable, and therefore may have a promising prospect in clinical application.