[Effects of salinomycin on proliferative, migratory and invasive property of tongue squamous cell carcinoma cells in vitro].

PURPOSE: To investigate the effects of salinomycin on proliferative, migratory and invasive properties of tongue squamous cell carcinoma cells, and to explore its possible mechanism.

METHODS

CCK8 assay was used to detect the effect of salicamycin and cisplatin on proliferative abilities of CAL-27 cells and EA.hy926 cells. The invasive and migratory ability was detected by Transwell assay. The protein expressions of E-cadherin, vimentin and β-catenin was evaluated by Western blot. SPSS20.0 software package was used to analyze the data.

RESULTS

Salicamycin can effectively inhibit proliferation of CAL-27 cells, and the inhibitive ability of salicamycin on the proliferation was stronger than that of cisplatin. CAL-27 cells were treated by salinomycin (4 μmol/L) before invasive and migratory abilities were examined. Compared with control group, the number of invasive and migratory cells in the salinomycin-treated group was significantly decreased (P＜0.05). Western blot analysis showed that the protein expression of vimentin and β-catenin was significantly down-regulated. The expression of E-cadherin was significantly increased with the increase of salicamycin concentration.

CONCLUSIONS

The proliferative, invasive and migratory ability of CAL-27 cells can be inhibited by salinomycin, which may be related to the inhibition of epithelial-mesenchymal transitions.