Vox sanguinis · 2025
Bacterial screening of platelet components: Experience at a hospital-based blood bank.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Al-Riyami Arwa Z, Al-Ruzeiqi Y, Al-Omairi I, Al-Muharrmi Z
Аннотация
BACKGROUND AND OBJECTIVES: Bacterial contamination of platelets stored at room temperature poses the highest risk of infection and fatality. Our hospital-based blood bank performs bacterial culture using the Bactec™ FX system for all whole blood-derived and apheresis units. Approximately 1 mL is collected from each of three to four buffy coat whole blood-derived platelet concentrates (PCs). The pooled sample is then inoculated into a single aerobic paediatric culture bottle. For apheresis units, 3 mL is inoculated per unit. Inoculation is performed at least 12 h after donation or 16-24 h after processing, when feasible. This study aims to determine the prevalence of culture-positive PCs.
MATERIALS AND METHODS: A retrospective review of the blood bank's records from April 2021 to August 2023 was carried out. The number of positive platelet cultures, identified organisms and their clinical significance were analysed. Clinical records were reviewed for any reported transfusion reactions.
RESULTS: A total of 5009 PCs were cultured. The rate of initial positive Bactec™ FX system results of pooled samples was 1.7%, corresponding to 85 PCs. Staphylococcus species accounted for 48% of the identified organisms. Upon repeat testing from individual PCs, five units (1:1000 units) were confirmed to be positive: four with Micrococcus luteus and one with Gram-positive bacilli. No clinical sequelae were reported in the recipients.
CONCLUSION: This study demonstrates a low platelet culture positivity rate, with the most common contaminant being Staphylococcus species. Further studies are required to evaluate the feasibility of a large-volume delayed-sampling testing protocol in our setting.
MATERIALS AND METHODS: A retrospective review of the blood bank's records from April 2021 to August 2023 was carried out. The number of positive platelet cultures, identified organisms and their clinical significance were analysed. Clinical records were reviewed for any reported transfusion reactions.
RESULTS: A total of 5009 PCs were cultured. The rate of initial positive Bactec™ FX system results of pooled samples was 1.7%, corresponding to 85 PCs. Staphylococcus species accounted for 48% of the identified organisms. Upon repeat testing from individual PCs, five units (1:1000 units) were confirmed to be positive: four with Micrococcus luteus and one with Gram-positive bacilli. No clinical sequelae were reported in the recipients.
CONCLUSION: This study demonstrates a low platelet culture positivity rate, with the most common contaminant being Staphylococcus species. Further studies are required to evaluate the feasibility of a large-volume delayed-sampling testing protocol in our setting.