Annals of the rheumatic diseases · 2009
Cell-mediated immune responses to influenza vaccination in Wegener's granulomatosis.
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Holvast Albert, de Haan Aalzen, van Assen Sander, Stegeman Coen A, Huitema Minke G, Huckriede Anke, Benne Cornelis A, Westra Johanna, Palache Abraham, Wilschut Jan, Kallenberg Cees G M, Bijl Marc
Аннотация
BACKGROUND: Both antibody and cell-mediated immune responses are involved in the defence against influenza. In Wegener's granulomatosis (WG), antibody responses to influenza vaccination appear to be similar to those in healthy controls, but cell-mediated responses have not been studied.
OBJECTIVE: To determine whether cell-mediated responses to influenza vaccination in WG vary from those in controls.
METHODS: Twenty-five patients with WG and healthy controls received subunit influenza vaccine. Peripheral blood mononuclear cells were obtained before and 1 month after vaccination. Cell-mediated responses to A/H1N1 and A/H3N2 were assessed using interferon gamma (IFN gamma) ELISpot and intracellular cytokine staining for IFN gamma, tumour necrosis factor and interleukin 2.
RESULTS: Before vaccination, patients and controls showed similar recall responses to A/H1N1 and A/H3N2. After vaccination, patients and controls showed similar levels of increase in spot-forming cells against A/H1N1 and A/H3N2. By flow cytometry, upon vaccination, proportions of cytokine-producing CD4 T cells increased in patients and controls for A/H1N1 and A/H3N2.
CONCLUSIONS: Cell-mediated responses to influenza vaccination in patients with WG are comparable to those in healthy controls.
OBJECTIVE: To determine whether cell-mediated responses to influenza vaccination in WG vary from those in controls.
METHODS: Twenty-five patients with WG and healthy controls received subunit influenza vaccine. Peripheral blood mononuclear cells were obtained before and 1 month after vaccination. Cell-mediated responses to A/H1N1 and A/H3N2 were assessed using interferon gamma (IFN gamma) ELISpot and intracellular cytokine staining for IFN gamma, tumour necrosis factor and interleukin 2.
RESULTS: Before vaccination, patients and controls showed similar recall responses to A/H1N1 and A/H3N2. After vaccination, patients and controls showed similar levels of increase in spot-forming cells against A/H1N1 and A/H3N2. By flow cytometry, upon vaccination, proportions of cytokine-producing CD4 T cells increased in patients and controls for A/H1N1 and A/H3N2.
CONCLUSIONS: Cell-mediated responses to influenza vaccination in patients with WG are comparable to those in healthy controls.