Bile Acids Modulate Hepatic Glycolipid Metabolism via the Microbiota-Gut-Liver Axis in Lambs.

BACKGROUND

Bile acids are essential molecules that facilitate lipid emulsification and function as signaling molecules mediating host-microbiota interactions. They shape the gut microbial structure and function, playing a critical role in metabolic regulation via the gut-liver axis.

OBJECTIVES

This study aimed to investigate the effects of exogenous bile acids, primarily hyocholic acid (HCA), on the microbiota-gut-liver metabolism in male Tan-lambs fed a high-grain diet.

METHODS

Thirty six-mo-old male Tan lambs (Ovis aries) were randomly allocated into either a control group or an HCA-supplemented group (n = 15 per group). The trial lasted 84 d, including a 14-d adaptation period. On day 70, 6 lambs from each group were randomly selected for slaughtering. Rumen and ileal contents were collected for microbial profiling via 16S rRNA sequencing, and liver tissue samples were harvested for transcriptomic and metabolomic analyses.

RESULTS

The HCA intervention significant altered the composition and structure of ruminal and ileal bacteria. Notable increases were observed in Turicibacter species [linear discriminant analysis (LDA) score = 2.48; P < 0.05] and Muribaculaceae (LDA score = 3.75; P < 0.05) in the rumen, and Eubacterium fissicatena group (LDA score = 2.50; P < 0.05) in the ileum. Key hepatic genes and metabolites targeted by HCA were identified, including ENPP3, RFK, Ifi203, LIPG, CYP1A1, and CYP4A11, nordeoxycholic acid (log-fold change = 6.30; P < 0.005), α-muricholic acid (log-fold change = 5.60; P < 0.001), and β-muricholic acid (log-fold change = 5.60; P < 0.001).

CONCLUSIONS

Exogenous bile acids regulate the microbiota-gut-liver axis, influencing hepatic glycolipid metabolism in sheep. Specifically, nordeoxycholic acid demonstrates potential as a dietary intervention to promote metabolic homeostasis in ruminants. These findings highlight the potential of HCA and nordeoxycholic acid as functional feed additives or prebiotic agents for improving metabolic health in ruminants and potentially other species.