Evaluation of caries prevention effect of dental dentin remineralization system constructed by biomimetic peptides and NACP: Molecular properties of calcium phosphate composite material NACP.

This study evaluated the anti-caries effect of the dentin remineralization system constructed by biomimetic peptide and calcium phosphate composite NACP, especially the molecular properties of NACP and its role in dentin remineralization, as well as the effectiveness and biocompatibility of this composite system in promoting dentin remineralization. The influence of DGP polypeptide and NACP bonding system on bonding strength was compared. Then, the NACP+DGP polypeptide system was constructed to evaluate its acid buffering ability and calcium and phosphorus ion release characteristics in vitro. In animal experiments, a rat model of secondary caries was established and biocompatibility was evaluated. The anti-caries effect was evaluated by microCT analysis and modified Keyes score. NACP materials show good micro-tensile strength and significant acid buffered capacity, which can effectively release calcium and phosphorus ions and promote biomimetic remineralization of dentin. In a pH cycle model in vitro, the NACP+DGP system significantly improved the microhardness of demineralized dentin and showed microscopic characteristics of remineralization in scanning electron microscopy (SEM) analysis. The results of animal experiments show that the system has remarkable preventive effect on secondary caries and good biocompatibility. Therefore, the dentin remineralization system based on calcium phosphate composite NACP shows good anti-caries effect, has strong biocompatibility and the potential to promote dentin remineralization, suggesting that the combination of this bionic peptide and NACP provides a new strategy and perspective for tooth caries prevention.