Prevalence of Giardia species in stool samples by ELISA in household cats from Romania and risk factors.

Stool samples (n=183) collected in Romania from cats of different ages, gender, breed, living conditions and origin were analysed by enzyme-linked immunosorbent assay using a commercial kit (Giardia Microwell ELISA, SafePath Laboratories, Carlsbad, USA). Fifty-one cats (27.9%) presented Giardia duodenalis antigens. The prevalence was significantly higher in cats with diarrhoea (32%, 16/50; P=0.04) and in cats from the north-west region (36.7%, 29/79; P=0.05). Young age (up to 6 months) was identified as the risk factor for infection (OR=0.29, 95% CI 0.09-0.92; P=0.03). There weren't any significant differences associated with gender, breed, medium, lifestyle, associated parasite infections, anthelmintic treatments, type of food or season.