Association of -429T/C RAGE Gene Polymorphism in Type 2 Diabetes Mellitus Patients with Diabetic Nephropathy in South Indian Population.

BACKGROUND

DN is a major microvascular complication of T2DM. Genetic factors, particularly polymorphisms in the RAGE gene, have been implicated in DN susceptibility.

Objective

This study aimed to evaluate the association of the -429T/C RAGE gene polymorphism with DN in T2DM patients from South India.

MATERIALS AND

METHODS

150 subjects, including HC, T2DM patients without nephropathy, and T2DM patients with DN, were enrolled. Genotyping of the -429T/C polymorphism was performed using PCR-RFLP with BsmFI enzyme.

RESULTS

The frequency of the TT genotype decreased progressively from 78% in HC to 58% in T2DM and 42% in DN. Conversely, the CC genotype increased from 8% in HC to 22% in T2DM and 28% in DN. The C allele frequency also rose from 0.15 in HC to 0.43 in DN. These differences were statistically significant. Discussion and.

CONCLUSION

The -429T/C RAGE gene polymorphism is significantly associated with DN in South Indian T2DM patients. The presence of the C allele appears to increase susceptibility to DN, whereas the TT genotype may confer a protective effect.