Iranian journal of basic medical sciences · 2025
Testicular extracellular matrix/gelatin-based scaffold using gas foaming to support spermatogonial stem cells.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Momeni Maryam, Ashtiani Mohammad Kazemi, Bashiri Zahra, Bagher Zohreh, Asgari Hamidreza, Koruji Morteza
Аннотация
OBJECTIVES: Developing a bioactive testicular scaffold has been proposed as a potential option to preserve male fertility. Using the gas foaming method, this study aimed to fabricate an effective and highly porous scaffold derived from a gelatin-testicular extracellular matrix.
MATERIALS AND METHODS: Male ram testis decellularization was performed using a combination of NaCl buffer and Triton. Then, evaluations were done for 4',6-diamidino-2-phenylindole, hematoxylin-eosin staining, and quantitative DNA content. Masson's trichrome, Alcian blue, and orcein staining were conducted to ensure the maintenance of ECM components post-decellularization. Porous scaffolds were fabricated using gelatin incorporation with various concentrations of extracted ECM via the gas foaming method. The mechanical and structural characteristics of the scaffolds, along with evaluations of cell spreading and penetration depth, were performed. Furthermore, scaffolds were used to culture mouse spermatogonial stem cells to investigate the morphology, viability, and adhesion of the cells on the scaffolds.
RESULTS: Our results showed successful decellularization of testicular tissue, resulting in significant removal of DNA content while preserving ECM major components. The hybrid scaffolds exhibited uniform porous microstructures with a pore size average ranging from 298-330 μm. There were no significant differences in biodegradation and swelling ratios between the scaffolds. The cell penetration index significantly increased in gel-ECM by 5% compared to other groups. Also, ECM5% led to increased cell attachment and viability, proper compressive strength, and a decrease in Young's module.
CONCLUSION: Our study suggests that using a combination of testicular ECM and gelatin shows promise in constructing bioartificial testes through the gas-foaming method.
MATERIALS AND METHODS: Male ram testis decellularization was performed using a combination of NaCl buffer and Triton. Then, evaluations were done for 4',6-diamidino-2-phenylindole, hematoxylin-eosin staining, and quantitative DNA content. Masson's trichrome, Alcian blue, and orcein staining were conducted to ensure the maintenance of ECM components post-decellularization. Porous scaffolds were fabricated using gelatin incorporation with various concentrations of extracted ECM via the gas foaming method. The mechanical and structural characteristics of the scaffolds, along with evaluations of cell spreading and penetration depth, were performed. Furthermore, scaffolds were used to culture mouse spermatogonial stem cells to investigate the morphology, viability, and adhesion of the cells on the scaffolds.
RESULTS: Our results showed successful decellularization of testicular tissue, resulting in significant removal of DNA content while preserving ECM major components. The hybrid scaffolds exhibited uniform porous microstructures with a pore size average ranging from 298-330 μm. There were no significant differences in biodegradation and swelling ratios between the scaffolds. The cell penetration index significantly increased in gel-ECM by 5% compared to other groups. Also, ECM5% led to increased cell attachment and viability, proper compressive strength, and a decrease in Young's module.
CONCLUSION: Our study suggests that using a combination of testicular ECM and gelatin shows promise in constructing bioartificial testes through the gas-foaming method.