Analytica chimica acta · 2025
Low-potential pyrene-coordinated MOFs and CoSOH nanosheets: An electrochemiluminescence energy resonance transfer system for aflatoxin B1 detection.
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Tan Cheng, Nie Jiayuan, Xi Jingjing, Mei Xiao, Wu Kang, Deng Anping, Feng Xinjian, Li Jianguo
Аннотация
BACKGROUND: Of the mycotoxins, aflatoxin is the most significant. The detection of aflatoxin B1 (AFB1) is crucial for ensuring food safety, as this highly carcinogenic toxin readily contaminates crops such as grains and nuts, and timely detection can effectively prevent associated health risks. The selection of luminophores is of paramount importance in the detection of ECL (electrochemiluminescence). Tetraphenylethylene (TPE) and its derivatives are the most representative molecules in aggregation-induced electrochemiluminescence. The energy resonance transfer phenomenon in ECL-RET (electrochemiluminescence resonance energy transfer) is a relatively novel ECL biosensing strategy.
RESULTS: Pyrene-based In-MOF with coordination-induced electrochemiluminescence (CIECL) properties were synthesized using In3+ and 1,3,6,8-tetrakis(4-carboxyphenyl) pyrene. CoSOH nanosheets exhibit extensive UV absorption and superior quenching capability for In-MOF. Hereby, In-MOF were employed as donor in an energy resonance transfer system, with CoSOH nanosheets serving as the acceptor, to construct an electrochemiluminescence immunosensor for detecting AFB1 in agricultural products. In-MOF exhibited significantly stronger ECL properties compared to the H4TBAPy monomer and aggregates due to the coordination-induced effect. The immunosensor, operating within the extremely low potential range from 0 to 0.65 V, effectively minimized interference from reductive species. By optimizing parameters such as the concentrations of CoSOH, antigens, antibodies, and the co-reactant DABCO, the sensor achieved a detection limit of 0.42 pg mL-1 for AFB1 with a linear range from 0.002 to 1000 ng mL-1.
SIGNIFICANCE: The immunosensor, operating within the extremely low potential range of 0-0.65 V, effectively minimized interference and preserved biological activity. It showed high selectivity and stability in real-sample detection, providing a simple method for AFB1 monitoring in crops, while expanding ECL-RET system applications.
RESULTS: Pyrene-based In-MOF with coordination-induced electrochemiluminescence (CIECL) properties were synthesized using In3+ and 1,3,6,8-tetrakis(4-carboxyphenyl) pyrene. CoSOH nanosheets exhibit extensive UV absorption and superior quenching capability for In-MOF. Hereby, In-MOF were employed as donor in an energy resonance transfer system, with CoSOH nanosheets serving as the acceptor, to construct an electrochemiluminescence immunosensor for detecting AFB1 in agricultural products. In-MOF exhibited significantly stronger ECL properties compared to the H4TBAPy monomer and aggregates due to the coordination-induced effect. The immunosensor, operating within the extremely low potential range from 0 to 0.65 V, effectively minimized interference from reductive species. By optimizing parameters such as the concentrations of CoSOH, antigens, antibodies, and the co-reactant DABCO, the sensor achieved a detection limit of 0.42 pg mL-1 for AFB1 with a linear range from 0.002 to 1000 ng mL-1.
SIGNIFICANCE: The immunosensor, operating within the extremely low potential range of 0-0.65 V, effectively minimized interference and preserved biological activity. It showed high selectivity and stability in real-sample detection, providing a simple method for AFB1 monitoring in crops, while expanding ECL-RET system applications.