[Knockdown of lncRNA SNHG6 inhibites the proliferation and epithelial mesenchymal transition in tongue cancer cells].

Objective To investigate the expression of lncRNA SNHG6 in tongue cancer and Tca1183 cells, and its effect on the biological characteristics of tongue cancer cells. Methods SNHG6 expression levels in tongue cancer tissue, normal tissue, normal tongue cells and cancer cells were detected by real time quantitative PCR to analyze the correlation between SNHG6 expression and clinicopathological characteristics of tongue cancer. SNHG6 interference plasmid was constructed and transfected into Tca1183 cells. MTT assay was used to detect the viability of Tca1183 cells. Colony formation assay was used to measure the proliferation of Tca1183 cells. The apoptosis of Tca1183 cells was detected by flow cytometry. Western blot analysis was used to evaluate the epithelial mesenchymal transition (EMT) related protein levels of β-catenin, E-cadherin, N-cadherin and vimentin. Results Compared to normal tissue or normal tongue epithelial cells, SNHG6 expression levels increased significantly in tongue cancer tissue or Tca1183 cells. SNHG6 expression in tongue cancer tissue was not correlated with age and gender, but with tissue differentiation degree, clinical stage and lymph node metastasis. Knockdown of SNHG6 in Tca1183 cells significantly inhibited the cell viability and proliferation and induced apoptosis. Furthermore, knockdown of SNHG6 significantly inhibited the EMT of Tca1183 cells by inducing the β-catenin and E-cadherin protein expression and inhibiting N-cadherin and vimentin protein expression. Conclusion SNHG6 expression is significantly increased in tongue cancer, and the interference of SNHG6 expression can inhibit the proliferation and EMT of tongue cancer cells.