Connective tissue research · 2010
The associations of circulating CD4+CD25high regulatory T cells and TGF-β with disease activity and clinical course in patients with adult-onset Still's disease.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Chen Der-Yuan, Chen Yi-Ming, Chen Hsin-Hua, Hsieh Chia-Wei, Lin Chi-Chen, Lan Joung-Liang
Аннотация
OBJECTIVE: To determine circulating levels of CD4(+)CD25(high) regulatory T (Treg) cells and transforming growth factor-β (TGF-β) in patients with adult-onset Still's disease (AOSD) and to examine the associations with disease activity and clinical course of this disease.
METHODS: The frequencies of circulating CD4(+)CD25(high) Treg cells in 52 active AOSD patients, 42 active systemic lupus erythematosus (SLE) patients, and 22 healthy controls (HCs) were determined using flow cytometry analysis. Levels of serum TGF-β and soluble interleukin-2 receptor (sIL-2R) were measured by enzyme-linked immunosorbent assay.
RESULTS: Significantly lower levels of circulating CD4(+)CD25(high) Treg cells and serum TGF-β were found in AOSD patients and SLE patients than those found in HCs. Levels of circulating CD4(+)CD25(high) Treg cells and TGF-β were inversely correlated with disease activity scores for AOSD patients and SLE patients. Circulating CD4(+)CD25(high) Treg cell frequencies were positively correlated with serum TGF-β levels for patients with both diseases. Levels of circulating CD4(+)CD25(high) Treg cells and TGF-β significantly increased, paralleling clinical remission and the decrease in levels of C-reactive protein and soluble interleukin-2 receptor after effective therapy in AOSD patients. AOSD patients with monocyclic course had significantly higher levels of circulating CD4(+)CD25(high) Treg cells and TGF-β compared to those with polycyclic and chronic articular course.
CONCLUSION: Diminished levels of circulating CD4(+)CD25(high) Treg cells and TGF-β, and inverse correlation with disease activity in patients with AOSD and SLE might be involved in the pathogenesis of both diseases. Increased levels of circulating CD4(+)CD25(high) Treg cells or TGF-β might be associated with a favorable clinical course in AOSD patients.
METHODS: The frequencies of circulating CD4(+)CD25(high) Treg cells in 52 active AOSD patients, 42 active systemic lupus erythematosus (SLE) patients, and 22 healthy controls (HCs) were determined using flow cytometry analysis. Levels of serum TGF-β and soluble interleukin-2 receptor (sIL-2R) were measured by enzyme-linked immunosorbent assay.
RESULTS: Significantly lower levels of circulating CD4(+)CD25(high) Treg cells and serum TGF-β were found in AOSD patients and SLE patients than those found in HCs. Levels of circulating CD4(+)CD25(high) Treg cells and TGF-β were inversely correlated with disease activity scores for AOSD patients and SLE patients. Circulating CD4(+)CD25(high) Treg cell frequencies were positively correlated with serum TGF-β levels for patients with both diseases. Levels of circulating CD4(+)CD25(high) Treg cells and TGF-β significantly increased, paralleling clinical remission and the decrease in levels of C-reactive protein and soluble interleukin-2 receptor after effective therapy in AOSD patients. AOSD patients with monocyclic course had significantly higher levels of circulating CD4(+)CD25(high) Treg cells and TGF-β compared to those with polycyclic and chronic articular course.
CONCLUSION: Diminished levels of circulating CD4(+)CD25(high) Treg cells and TGF-β, and inverse correlation with disease activity in patients with AOSD and SLE might be involved in the pathogenesis of both diseases. Increased levels of circulating CD4(+)CD25(high) Treg cells or TGF-β might be associated with a favorable clinical course in AOSD patients.