Zhen ci yan jiu = Acupuncture research · 2008
[Correlation between the therapeutic efficacy of electroacupuncture of ear-body acupoints and mast cells in experimental gastric ulcer rats].
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He Jun-Na, Luo Ming-Fu, Guo Ying, Li Cui-Hong, Zhang Jin-Ling
Аннотация
OBJECTIVE: To study the interrelation between the therapeutic efficacy of electroacupuncture (EA) and mast cells (MC) in experimental gastric ulcer rats.
METHODS: Thirty Wistar rats were randomly divided into normal control group, model group and EA group, with 10 cases in each. Experimental gastric ulcer model was established by injecting 20% glacial acetic acid (0.05 ml) into the tissue between the muscular layer and the placenta percreta of the gastric anterior wall in the gastric antrum under anesthesia. EA (2/15 Hz, 1 mA) was applied to bilateral "Wei" (Stomach, an otopoint) and "Zusanli" (ST 36) for 30 min, once daily for 7 days. The tissues of these two acupoints were sampled to be stained with acetylcholine esterase (AChE) and toluidine blue (TB) respectively (counterstaining) after section (15 microm). The gastric ulcer tissue was stained with H&E method
RESULTS: Compared with control group, the number and degranulation ratios of MC in the dermis of "Wei"-point and ST36 region in model group and EA group increased significantly (P<0.01); while in comparison with model group, both the number and the degranulation ratio of MC in EA group decreased considerably (P<0.01). H&E stained tissues showed smooth gastric mucosal membrane in control group, ulcerative injury involving the muscular layer in model group, and mild injury in EA group.
CONCLUSION: EA can suppress the degranulation of MC in "Wei"-point and ST36 regions and effectively relieve ulcerative injury in gastric ulcer rats. There exists a certain degree of interrelation among the degranulation ratio of MC, gastric ulcerative injury, and the therapeutic efficacy.
METHODS: Thirty Wistar rats were randomly divided into normal control group, model group and EA group, with 10 cases in each. Experimental gastric ulcer model was established by injecting 20% glacial acetic acid (0.05 ml) into the tissue between the muscular layer and the placenta percreta of the gastric anterior wall in the gastric antrum under anesthesia. EA (2/15 Hz, 1 mA) was applied to bilateral "Wei" (Stomach, an otopoint) and "Zusanli" (ST 36) for 30 min, once daily for 7 days. The tissues of these two acupoints were sampled to be stained with acetylcholine esterase (AChE) and toluidine blue (TB) respectively (counterstaining) after section (15 microm). The gastric ulcer tissue was stained with H&E method
RESULTS: Compared with control group, the number and degranulation ratios of MC in the dermis of "Wei"-point and ST36 region in model group and EA group increased significantly (P<0.01); while in comparison with model group, both the number and the degranulation ratio of MC in EA group decreased considerably (P<0.01). H&E stained tissues showed smooth gastric mucosal membrane in control group, ulcerative injury involving the muscular layer in model group, and mild injury in EA group.
CONCLUSION: EA can suppress the degranulation of MC in "Wei"-point and ST36 regions and effectively relieve ulcerative injury in gastric ulcer rats. There exists a certain degree of interrelation among the degranulation ratio of MC, gastric ulcerative injury, and the therapeutic efficacy.