Neuroimmunomodulation · 2011
Expression of Toll-like receptors 2, 4 and 9 in patients with Guillain-Barré syndrome.
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Wang Yu-Zhong, Liang Qiu-Hua, Ramkalawan Hhoonisha, Wang Yan-Li, Yang Yan-Fang, Zhou Wen-Bin, Tian Fa-Fa, Li Jing, Yang Huan
Аннотация
OBJECTIVE: A myriad of transcription factors and inflammatory cytokines have been described to participate in the pathogenesis of Guillain-Barré syndrome (GBS). However, the innate immunity components--Toll-like receptors (TLRs)--have never been explored in this disease. We therefore investigated the expression of TLR2, 4 and 9 in the peripheral circulation of GBS patients as well as healthy controls.
METHODS: Twenty-one GBS patients and 21 healthy donors participated in this study. Peripheral blood mononuclear cells were used for mRNA and protein analysis of TLR-related molecules. Also, peripheral blood mononuclear cells from different subjects were incubated with different TLR agonists and the subsequent IFN-γ secretion was determined.
RESULTS: Expression of TLR2, 4 and 9 as well as their related signaling molecules were higher in GBS patients compared to healthy controls. Disability scores of GBS patients had a strong positive correlation with the high levels of expression of TLR2, 4 and 9.
CONCLUSIONS: The TLR signaling pathway may be involved in the pathogenesis of GBS.
METHODS: Twenty-one GBS patients and 21 healthy donors participated in this study. Peripheral blood mononuclear cells were used for mRNA and protein analysis of TLR-related molecules. Also, peripheral blood mononuclear cells from different subjects were incubated with different TLR agonists and the subsequent IFN-γ secretion was determined.
RESULTS: Expression of TLR2, 4 and 9 as well as their related signaling molecules were higher in GBS patients compared to healthy controls. Disability scores of GBS patients had a strong positive correlation with the high levels of expression of TLR2, 4 and 9.
CONCLUSIONS: The TLR signaling pathway may be involved in the pathogenesis of GBS.
Предметные рубрики MeSH
AdultCytokinesDose-Response Relationship, DrugEnzyme-Linked Immunosorbent AssayFemaleGene Expression RegulationGuillain-Barre SyndromeHumansLeukocytes, MononuclearLipopolysaccharidesMaleMiddle AgedRNA, MessengerSignal TransductionStatistics as TopicToll-Like Receptor 2Toll-Like Receptor 4Toll-Like Receptor 9Toll-Like ReceptorsYoung Adult