Journal of Ayub Medical College, Abbottabad : JAMC · 2011
Helicobacter pylori detection in chronic gastritis: a comparison of staining methods.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Ahmad Fiaz, Jaffar Rozina, Khan Inamullah
Аннотация
BACKGROUND: Helicobacter pylori is an important cause of chronic gastritis, gastric ulceration and gastric malignancies as gastric carcinoma and MALT lymphoma. Its definitive diagnosis is based on histopathology. Routine H & E stain is not very effective in its detection, immune-stains and fluorescent stains are costly. Need for simple cheap and sensitive stain has always been a topic of hot debate and extensive research.
METHOD: paraffin embedded blocks of all adult patients diagnosed as chronic gastritis/gastric ulceration with no accompanying gastric pathology as hypertrophic gastropathys, and neoplasias were taken into study. Three sections of 4 micron were cut and stained with routine H & E, Giemsa, and Cresyl fast violet.
RESULTS: Total number of patients was 50. Out of these 37 (74%) were males and 13 (26%) were females. Mean age of the patients was 50.4 years. Thirty-four percent (34%) were positive in normal H & E stain, 68% were positive in Giemsa and 76% were positive in Cresyl fast violet.
CONCLUSION: Cresyl fast violet is a good stain for diagnosis ofH. pylori gastritis.
METHOD: paraffin embedded blocks of all adult patients diagnosed as chronic gastritis/gastric ulceration with no accompanying gastric pathology as hypertrophic gastropathys, and neoplasias were taken into study. Three sections of 4 micron were cut and stained with routine H & E, Giemsa, and Cresyl fast violet.
RESULTS: Total number of patients was 50. Out of these 37 (74%) were males and 13 (26%) were females. Mean age of the patients was 50.4 years. Thirty-four percent (34%) were positive in normal H & E stain, 68% were positive in Giemsa and 76% were positive in Cresyl fast violet.
CONCLUSION: Cresyl fast violet is a good stain for diagnosis ofH. pylori gastritis.