In vivo (Athens, Greece) · 2010
An easy method to identify parietal cells in gastric biopsies.
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Rubio Carlos A
Аннотация
BACKGROUND: Sections from gastric biopsies are usually stained with hematoxylin and eosin (H&E), a stain that is not optimal for the recognition of many parietal cells. This paper describes a more suitable routine stain to identify parietal cells.
PATIENTS AND METHODS: Nineteen sets of gastric biopsies were consecutively stained with H&E and with modified Giemsa. Giemsa-stained fundic biopsies showed a parietal cell band intercalated between the fovelar epithelium and the chief glands. The continuity of this band was studied at x4 magnification and its thickness in one well-oriented field at x10 magnification.
RESULTS: A distinct, continuous parietal cell band was recorded in fundic biopsies exhibiting normal mucosa, acute gastritis or chronic gastritis without glandular atrophy (Group A). A discontinuous or lack of parietal cell band was found in fundic biopsies exhibiting chronic gastritis with glandular atrophy or with intestinal metaplasia (Group B). The ratio of parietal cell band/total mucosal thickness ranged between 0.30 and 0.40 in Group A and between 0 and 0.25 in Group B.
CONCLUSION: A parietal cell band was readily demonstrated in sections from gastric biopsies stained with Giemsa, but not in those stained with H&E. Discontinuity with reduced or absent band was recorded in gastric diseases characterised by a decrease of the parietal cell population.
PATIENTS AND METHODS: Nineteen sets of gastric biopsies were consecutively stained with H&E and with modified Giemsa. Giemsa-stained fundic biopsies showed a parietal cell band intercalated between the fovelar epithelium and the chief glands. The continuity of this band was studied at x4 magnification and its thickness in one well-oriented field at x10 magnification.
RESULTS: A distinct, continuous parietal cell band was recorded in fundic biopsies exhibiting normal mucosa, acute gastritis or chronic gastritis without glandular atrophy (Group A). A discontinuous or lack of parietal cell band was found in fundic biopsies exhibiting chronic gastritis with glandular atrophy or with intestinal metaplasia (Group B). The ratio of parietal cell band/total mucosal thickness ranged between 0.30 and 0.40 in Group A and between 0 and 0.25 in Group B.
CONCLUSION: A parietal cell band was readily demonstrated in sections from gastric biopsies stained with Giemsa, but not in those stained with H&E. Discontinuity with reduced or absent band was recorded in gastric diseases characterised by a decrease of the parietal cell population.