Ocular immunology and inflammation · 2013
Transplantation of CX3CL1-expressing mesenchymal stem cells provides neuroprotective and immunomodulatory effects in a rat model of retinal degeneration.
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Huang Libin, Xu Wei, Xu Guoxing
Аннотация
PURPOSE: To investigate the neuroprotective and immunomodulatory effects of mesenchymal stem cells (MSCs) engineered to secrete CX3CL1 on the light-injured retinal structure and function.
METHODS: Normal MSCs and CX3CL1-expressing MSCs (CX3CL1-MSCs) were transplanted into the subretinal space of light-injured rats. By ERG and TUNEL methods, their rescue effect of the host retina was compared with untreated light-injured and vehicle-injected rats. Activated microglia in the retina were stained by ED-1 antibody, and Western blot was performed to quantify cytokines secreted by the retina post-transplantation.
RESULTS: ERG analysis showed better function in CX3CL1-MSC-injected group than other groups at 21 days after transplantation (p < 0.05). CX3CL1-MSCs inhibited apoptosis of the retinal cells and microglial activation. Neurotrophic factors expression in host retina that received CX3CL1-MSCs was stronger than in the retina that received normal MSCs. Conversely, the expression of proinflammatory factors was downregulated.
CONCLUSIONS: CX3CL1-MSCs subretinal transplantation may enhance protective effect against light-induced retinal degeneration.
METHODS: Normal MSCs and CX3CL1-expressing MSCs (CX3CL1-MSCs) were transplanted into the subretinal space of light-injured rats. By ERG and TUNEL methods, their rescue effect of the host retina was compared with untreated light-injured and vehicle-injected rats. Activated microglia in the retina were stained by ED-1 antibody, and Western blot was performed to quantify cytokines secreted by the retina post-transplantation.
RESULTS: ERG analysis showed better function in CX3CL1-MSC-injected group than other groups at 21 days after transplantation (p < 0.05). CX3CL1-MSCs inhibited apoptosis of the retinal cells and microglial activation. Neurotrophic factors expression in host retina that received CX3CL1-MSCs was stronger than in the retina that received normal MSCs. Conversely, the expression of proinflammatory factors was downregulated.
CONCLUSIONS: CX3CL1-MSCs subretinal transplantation may enhance protective effect against light-induced retinal degeneration.
Предметные рубрики MeSH
AnimalsApoptosisBlotting, WesternCells, CulturedChemokine CX3CL1DNADisease Models, AnimalElectroretinographyGene Expression RegulationImmunohistochemistryImmunomodulationIn Situ Nick-End LabelingMaleMesenchymal Stem Cell TransplantationMesenchymal Stem CellsPhotoreceptor Cells, VertebratePolymerase Chain ReactionRatsRats, Sprague-DawleyRetinal Degeneration