Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2011
[Effects of CD74 gene on IFNγR gene expression in MG TEC].
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Li Qian-Ru, Zang Wen-Qiao, Gao Feng, Du Ying, Zhang Qing-Yong
Аннотация
AIM: To study the effects of CD74 gene on IFN-γR expression in myasthenia gravis thymic epithelial cell(TEC).
METHODS: PCMV-CD74 transiently transfect to primary cultured TEC mediated with lipofectamine, the result of transfection and some autoimmune related genes such as IFN-γR were detected by real-time RT-PCR.
RESULTS: Real-time quantitative RT-PCR results show that the mRNA expression level of IL-4R, IL-32, IFN-γR, ECGF1, HLA-A, HLA-DR increased significantly, with the high express ion of CD74 gene in thymic epithelial cells.
CONCLUSION: CD74 gene over-expression in TEC can increase IFN-γR mRNA expression.
METHODS: PCMV-CD74 transiently transfect to primary cultured TEC mediated with lipofectamine, the result of transfection and some autoimmune related genes such as IFN-γR were detected by real-time RT-PCR.
RESULTS: Real-time quantitative RT-PCR results show that the mRNA expression level of IL-4R, IL-32, IFN-γR, ECGF1, HLA-A, HLA-DR increased significantly, with the high express ion of CD74 gene in thymic epithelial cells.
CONCLUSION: CD74 gene over-expression in TEC can increase IFN-γR mRNA expression.