Results of a collaborative study on DNA identification of aged bone samples.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
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Vanek Daniel, Budowle Bruce, Dubska-Votrubova Jitka, Ambers Angie, Frolik Jan, Pospisek Martin, Al Afeefi Ahmed Anwar, Al Hosani Khalid Ismaeil, Allen Marie, Al Naimi Khudooma Saeed, Al Salafi Dina, Al Tayyari Wafa Ali Rashid, Arguetaa Wendy, Bottinelli Michel, Bus Magdalena M, Cemper-Kiesslich Jan, Cepil Olivier, De Cock Greet, Desmyter Stijn, El Amri Hamid, El Ossmani Hicham, Galdies Ruth, Grün Sebastian, Guidet Francois, Hoefges Anna, Iancu Cristian Bogdan, Lotz Petra, Maresca Alessandro, Nagy Marion, Novotny Jindrich, Rachid Hajar, Rothe Jessica, Stenersen Marguerethe, Stephenson Mishel, Stevanovitch Alain, Strien Juliane, Sumita Denilce R, Vella Joanna, Zander Judith
Аннотация
METHODS: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory.
RESULTS: Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality.
CONCLUSION: The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.