Nature communications · 2025
Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
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Sadaow Lakkhana, Rodpai Rutchanee, Smout Michael J, Nakajima Rie, Boonroumkaew Patcharaporn, Sotillo Javier, Tedla Bemnet A, Luvira Vor, Kitkhuandee Amnat, Paonariang Krisada, Sukeepaisarnjaroen Wattana, Yamasaki Hiroshi, Suttiprapa Sutas, Laha Thewarach, Sripa Banchob, de Assis Rafael, Jain Aarti, Ittiprasert Wannaporn, Mann Victoria H, Wong Yide, Felgner Philip L, Maleewong Wanchai, Brindley Paul J, Loukas Alex, Intapan Pewpan M
Аннотация
Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.