Zhonghua nei ke za zhi · 1999
[Apoptosis in parathyroid of uremic patients with secondary hyperparathyroidism and the influence of Ca2+ and calcitriol on APO].
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Zhang P, Wei J, Han D
Аннотация
OBJECTIVE: To examine whether the alteration of apoptosis is pre sent in parathyroid (PT) tissue of hyperparathyroidism (HPTH).
METHODS: DNA strand breaks were sought by TdT-mediated dUTP nick end-labeling assay (TUNEL) and DNA gel electrophoresis in 4 specimens of normal PT tissue, 7 of primary HPTH (primary HPTH) and 9 of secondary HPTH tissue (secondary HPTH).
RESULTS: The data showed that the percentage of apoptotic cells was (1.3 +/- 0.4) % in normal PT, (3.9 +/- 0.7) % in primary HPTH and (5.6 +/- 0.8) % in secondary HPTH (P < 0.04), primary HPTH vs normal PT; P < 0.001, secondary HPTH vs normal PT respectively. There was no significant difference in the apoptotic incidence between primary and secondary HPTH. In vitro study, a greater amount of apoptotic TUNEL-positive cells was found 24 hours after exposure to high Ca(2+) (1.9 mmol/L) than to low Ca(2+) (0.5 mmol/L) concentration and 96 hours after treatment with high calcitriol (10(-7)mol/L) than with low calcitriol (10(-11) mol/L) concentration. However, no DNA ladder feature was observed in electrophoresis of DNA extracted from cultured parathyroid cells of HPTH.
CONCLUSION: It was shown that apoptosis did not decrease, but actually enhanced, in hyperplastic parathyroid tissue of patients with primary or secondary HPTH and that a high extracellular concentration of either Ca(2+) or calcitriol was capable of enhancing apoptosis in human parathyroid cells of secondary HPTH complicating chronic renal failure.
METHODS: DNA strand breaks were sought by TdT-mediated dUTP nick end-labeling assay (TUNEL) and DNA gel electrophoresis in 4 specimens of normal PT tissue, 7 of primary HPTH (primary HPTH) and 9 of secondary HPTH tissue (secondary HPTH).
RESULTS: The data showed that the percentage of apoptotic cells was (1.3 +/- 0.4) % in normal PT, (3.9 +/- 0.7) % in primary HPTH and (5.6 +/- 0.8) % in secondary HPTH (P < 0.04), primary HPTH vs normal PT; P < 0.001, secondary HPTH vs normal PT respectively. There was no significant difference in the apoptotic incidence between primary and secondary HPTH. In vitro study, a greater amount of apoptotic TUNEL-positive cells was found 24 hours after exposure to high Ca(2+) (1.9 mmol/L) than to low Ca(2+) (0.5 mmol/L) concentration and 96 hours after treatment with high calcitriol (10(-7)mol/L) than with low calcitriol (10(-11) mol/L) concentration. However, no DNA ladder feature was observed in electrophoresis of DNA extracted from cultured parathyroid cells of HPTH.
CONCLUSION: It was shown that apoptosis did not decrease, but actually enhanced, in hyperplastic parathyroid tissue of patients with primary or secondary HPTH and that a high extracellular concentration of either Ca(2+) or calcitriol was capable of enhancing apoptosis in human parathyroid cells of secondary HPTH complicating chronic renal failure.