Clinical biochemistry · 2015
Plasma microRNAs serve as potential biomarkers for abdominal aortic aneurysm.
СтатьяПоддержка исследования: вне правительства СШАВалидационное исследованиеНазвание и аннотация: Перевод пока не подготовлен
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Zhang Wenwen, Shang Tao, Huang Chen, Yu Tong, Liu Chen, Qiao Tong, Huang Dian, Liu Zhao, Liu Changjian
Аннотация
OBJECTIVES: Abdominal aortic aneurysm (AAA) is often asymptomatic until rupture occurs. Although ultrasound screening has significantly improved the early diagnosis of AAA, the timely biomarker-based diagnosis of AAA remains a major clinical challenge. In this study, we aimed to assess plasma microRNAs (miRNAs) as promising novel biomarkers in patients with AAA.
METHODS: Pooled plasma samples from 10 AAA and 10 healthy controls were profiled by microarray. The differentially expressed miRNAs were evaluated in a separate cohort of 120 subjects, including 60 AAA patients and 60 normal controls.
RESULTS: The initial profiling study identified 151 miRNAs that showed more than two-fold change. Among them, three miRNAs, miR-191-3p, miR-455-3p and miR-1281 exhibited the largest increase in the patient group (fold change>5). A subsequent validation study confirmed the elevation of these three miRNAs. Receiver operator characteristic curve analysis using the expression ratio of miR-191-3p, miR-455-3p and miR-1281 showed an area under the curve of 0.9700, 0.9825 and 0.9206, respectively.
CONCLUSION: Our results suggest that plasma miR-191-3p, miR-455-3p and miR-1281 may be used as potential diagnosis biomarkers for AAA.
METHODS: Pooled plasma samples from 10 AAA and 10 healthy controls were profiled by microarray. The differentially expressed miRNAs were evaluated in a separate cohort of 120 subjects, including 60 AAA patients and 60 normal controls.
RESULTS: The initial profiling study identified 151 miRNAs that showed more than two-fold change. Among them, three miRNAs, miR-191-3p, miR-455-3p and miR-1281 exhibited the largest increase in the patient group (fold change>5). A subsequent validation study confirmed the elevation of these three miRNAs. Receiver operator characteristic curve analysis using the expression ratio of miR-191-3p, miR-455-3p and miR-1281 showed an area under the curve of 0.9700, 0.9825 and 0.9206, respectively.
CONCLUSION: Our results suggest that plasma miR-191-3p, miR-455-3p and miR-1281 may be used as potential diagnosis biomarkers for AAA.
Предметные рубрики MeSH
AgedAortic Aneurysm, AbdominalBiomarkersCase-Control StudiesCohort StudiesFemaleHumansMaleMicroRNAsMiddle AgedMolecular Diagnostic TechniquesOligonucleotide Array Sequence AnalysisPlasmaROC CurveReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityUp-Regulation