International journal of reproductive biomedicine · 2025
Chrysin's protective effect on the expression of protamine, Tsga10, Dazl, and Akap4 genes against diazinon toxin in male rats: An experimental study.
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Mojibi Fatemeh, Fattahi Esmail, Jorsaraei Seyed Gholam Ali, Kazemi Sohrab, Tabari Maryam Gholamitabar
Аннотация
BACKGROUND: Environmental pollution due to pesticides is a major health problem worldwide that causes toxicity. Chrysin (C) is a flavonoid that reduces the adverse effects caused by diazinon (D).
OBJECTIVE: To investigate the protective effects of C on the expression of protamine, testis-specific gene antigen 10 (Tsga10), deleted azoospermia-like gene (Dazl), and A-Kinase anchoring protein 4 (Akap4) genes in the testicular tissue of rats receiving D.
MATERIALS AND METHODS: In this experimental study, 42 Wistar rats (7-8 wk, 180-250 gr) were selected. This study was conducted in 2021 at the Pasteur Institute of Iran, Amol Research Center, Amol, Iran. Injections were performed 5 days a week for 4 wk. Random grouping of rats: 1) control: without any injection. 2) Sham: injection of 10% tween 80 solution as D solvent. 3) D: injection at a concentration of 20 mg/kg/BW. 4 and 5) C with low dose (C10 mg/kg/BW) and high dose (C20 mg/kg/BW). 6 and 7) D with low dose of C (D20 mg/kg/BW/ C10 mg/kg/BW) and D with high dose: (D20 mg/kg/BW/ C20 mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression.
RESULTS: Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression of Dazl (p < 0.0001), Tsga10 (p = 0.01), and protamine (p < 0.0001) genes, but increased the expression of Akap4 gene (p < 0.0001).
CONCLUSION: D has a negative effect on the expression of meiotic genes in rat testicular tissue, while C greatly reduces the adverse effects caused by D.
OBJECTIVE: To investigate the protective effects of C on the expression of protamine, testis-specific gene antigen 10 (Tsga10), deleted azoospermia-like gene (Dazl), and A-Kinase anchoring protein 4 (Akap4) genes in the testicular tissue of rats receiving D.
MATERIALS AND METHODS: In this experimental study, 42 Wistar rats (7-8 wk, 180-250 gr) were selected. This study was conducted in 2021 at the Pasteur Institute of Iran, Amol Research Center, Amol, Iran. Injections were performed 5 days a week for 4 wk. Random grouping of rats: 1) control: without any injection. 2) Sham: injection of 10% tween 80 solution as D solvent. 3) D: injection at a concentration of 20 mg/kg/BW. 4 and 5) C with low dose (C10 mg/kg/BW) and high dose (C20 mg/kg/BW). 6 and 7) D with low dose of C (D20 mg/kg/BW/ C10 mg/kg/BW) and D with high dose: (D20 mg/kg/BW/ C20 mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression.
RESULTS: Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression of Dazl (p < 0.0001), Tsga10 (p = 0.01), and protamine (p < 0.0001) genes, but increased the expression of Akap4 gene (p < 0.0001).
CONCLUSION: D has a negative effect on the expression of meiotic genes in rat testicular tissue, while C greatly reduces the adverse effects caused by D.