Journal of endodontics · 2012
T-lymphocyte and cytokine expression in human inflammatory periapical lesions.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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de Brito Luciana Carla Neves, Teles Flávia Rocha Fonseca, Teles Ricardo Palmier, Totola Antônio Helvécio, Vieira Leda Quércia, Sobrinho Antônio Paulino Ribeiro
Аннотация
INTRODUCTION: Lymphocytes, among many cells, express different sets of cytokines, chemokines, and receptors, which are considered important mediators of periapical immune response to infection.
METHODS: The aim of this study was to evaluate the mRNA expression of CD4(+)CD28(+) and CD8(+) T genes and the gene expression of interferon-γ, tumor necrosis factor-α, interleukin (IL)-1β, IL-17A, IL-10, CCL2/MCP-1, CCL4, CCL5, CXCR4, CCR5, and receptor activator for nuclear factor kappa B ligand (RANKL) in periapical interstitial fluid from human root canal infections. The samples were collected immediately after root canal cleaning and 7 days later (restrained root canal bacterial load) to characterize those gene expressions.
RESULTS: Real-time polymerase chain reaction demonstrated significantly higher levels of CD4(+)CD28(+) and CD8(+) T-cell markers in the former root canal condition and an increase of IL-10 and CXCR4, followed by a decrease of proinflammatory cytokines such as RANKL, interferon-γ, IL-1β, and CCL5.
CONCLUSIONS: Analyses of T-lymphocyte and cytokine expression in periapical area were able to show that distinct root canal conditions might play regulatory roles in controlling local immune/inflammatory processes.
METHODS: The aim of this study was to evaluate the mRNA expression of CD4(+)CD28(+) and CD8(+) T genes and the gene expression of interferon-γ, tumor necrosis factor-α, interleukin (IL)-1β, IL-17A, IL-10, CCL2/MCP-1, CCL4, CCL5, CXCR4, CCR5, and receptor activator for nuclear factor kappa B ligand (RANKL) in periapical interstitial fluid from human root canal infections. The samples were collected immediately after root canal cleaning and 7 days later (restrained root canal bacterial load) to characterize those gene expressions.
RESULTS: Real-time polymerase chain reaction demonstrated significantly higher levels of CD4(+)CD28(+) and CD8(+) T-cell markers in the former root canal condition and an increase of IL-10 and CXCR4, followed by a decrease of proinflammatory cytokines such as RANKL, interferon-γ, IL-1β, and CCL5.
CONCLUSIONS: Analyses of T-lymphocyte and cytokine expression in periapical area were able to show that distinct root canal conditions might play regulatory roles in controlling local immune/inflammatory processes.
Предметные рубрики MeSH
Bacterial InfectionsCD28 AntigensCD4 AntigensCD4-Positive T-LymphocytesCD8 AntigensCD8-Positive T-LymphocytesChemokine CCL2Chemokine CCL4Chemokine CCL5CytokinesDental Pulp NecrosisExtracellular FluidFollow-Up StudiesHumansInflammation MediatorsInterferon-gammaInterleukin-10Interleukin-17Interleukin-1betaPeriapical PeriodontitisRANK LigandReceptors, CCR5Receptors, CXCR4Root Canal TherapyT-LymphocytesTumor Necrosis Factor-alpha