Journal of periodontology · 2011
S-nitrosoglutathione decreases inflammation and bone resorption in experimental periodontitis in rats.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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de Menezes Adriana Magalhães Andrade, de Souza Gabriela Freitas Pereira, Gomes Antoniella Souza, de Carvalho Leitão Renata Ferreira, Ribeiro Ronaldo de Albuquerque, de Oliveira Marcelo Ganzarolli, de Castro Brito Gerly Anne
Аннотация
BACKGROUND: S-nitrosoglutathione (GSNO) is a nitric oxide donor that may exert antioxidant, anti-inflammatory, and microbicidal actions and is thus a potential drug for the topical treatment of periodontitis. In this study, the effect of intragingival injections of GSNO-containing polyvinylpyrrolidone (PVP) formulations is evaluated in a rat model of periodontitis.
METHODS: Periodontal disease was induced by placing a sterilized nylon (000) thread ligature around the cervix of the second left upper molar of the animals, which received intragingival injections of PVP; saline; or PVP/GSNO solutions which corresponded to GSNO doses of 25, 100, and 500 nmol; 1 hour before periodontitis induction, and thereafter, daily for 11 days.
RESULTS: PVP/GSNO formulations at doses of 25 and/or 100, but not 500 nmol caused significant inhibition of alveolar bone loss, increase of bone alkaline phosphatase, decrease of myeloperoxidase activity, as well as significant reduction of inflammatory and oxidative stress markers when compared to saline and PVP groups. These effects were also associated with a decrease of matrix metalloproteinases 1 and 8, inducible nitric oxide synthase, and nuclear factor-κB immunostaining in the periodontium.
CONCLUSION: Local intragingival injections of GSNO reduces inflammation and bone loss in experimental periodontal disease.
METHODS: Periodontal disease was induced by placing a sterilized nylon (000) thread ligature around the cervix of the second left upper molar of the animals, which received intragingival injections of PVP; saline; or PVP/GSNO solutions which corresponded to GSNO doses of 25, 100, and 500 nmol; 1 hour before periodontitis induction, and thereafter, daily for 11 days.
RESULTS: PVP/GSNO formulations at doses of 25 and/or 100, but not 500 nmol caused significant inhibition of alveolar bone loss, increase of bone alkaline phosphatase, decrease of myeloperoxidase activity, as well as significant reduction of inflammatory and oxidative stress markers when compared to saline and PVP groups. These effects were also associated with a decrease of matrix metalloproteinases 1 and 8, inducible nitric oxide synthase, and nuclear factor-κB immunostaining in the periodontium.
CONCLUSION: Local intragingival injections of GSNO reduces inflammation and bone loss in experimental periodontal disease.
Предметные рубрики MeSH
Alkaline PhosphataseAlveolar Bone LossAlveolar ProcessAnimalsAnti-Inflammatory AgentsBiomarkersBone ResorptionDisease Models, AnimalGingivaInjectionsInterleukin-1betaLipid PeroxidationMatrix Metalloproteinase InhibitorsNF-kappa BNitric Oxide DonorsNitric Oxide Synthase Type IIOxidative StressPeriodontitisPeroxidasePharmaceutic AidsPovidoneRatsRats, WistarS-NitrosoglutathioneSodium ChlorideTumor Necrosis Factor-alpha