Revista da Sociedade Brasileira de Medicina Tropical · 2023
Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus.
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Fernandes Blenda, Santrer Emanoelle Fernandes Rutren La, Figueiredo Sônia Maria de, Rocha-Silva Fabiana, Assunção Cláudia Barbosa, Abreu Amanda Gabrielle, Santiago Iara Furtado, Johann Susana, Caligiorne Rachel Basques
Аннотация
BACKGROUND: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR.
METHODS: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique.
RESULTS: Three specific primers with 100% specificity for the Sporothrix genus were generated.
CONCLUSIONS: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.
METHODS: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique.
RESULTS: Three specific primers with 100% specificity for the Sporothrix genus were generated.
CONCLUSIONS: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.