Interaction between human oxoguanine glycosylase 1 gene polymorphisms and smoking status on nasopharyngeal carcinoma risk.

This study aims to evaluate the impact of four SNPs of human 8-oxoguanine DNA glycosylase 1 (hOGG1) gene, and its interaction with smoking and alcohol drinking on the risk of nasopharyngeal carcinoma (NPC). Hardy-Weinberg equilibrium (HWE) and the relationship between four SNPs of the hOGG1 gene and the risk of NPC were tested using the SNPStats online software ( https://www.snpstats.net/start.htm ). Generalized multifactor dimensionality reduction (GMDR) was utilized to screen the optimal interaction combinations among four hOGG1 gene SNPs, smoking, and alcohol drinking. We found that rs1052133- Cys allele was associated with increased NPC risk, ORs (95% CI) were 1.42 (1.12-1.85) for Ser/Cys genotype, 1.95 (1.51-2.48) for Cys/Cys genotype, 1.57 (1.18-2.05) for Ser/Cys or Cys/Cys genotype, compared to Ser/Ser genotype. We also found that rs159153- T allele was associated with increased NPC risk, ORs (95% CI) were 1.35 (1.06-1.73) for CT genotype, 2.18 (1.62-2.85) for TT genotype, 1.45 (1.09-1.90) for CT or TT genotype, compared to CC genotype. However, we did not find any statistically significant impact of the minor alleles of rs3219008 and rs293795 on the risk of NPC. GMDR model found a significant gene-environment interaction combination (two-locus model with P = 0.018) between rs1052133 and smoking. Compared to never smokers with rs1052133 Ser/Ser genotype, ever or currently smokers with rs1052133- Ser/Cys or Cys/Cys genotype have the highest NPC risk, OR (95% CI) = 3.18 (1.94-4.42). The rs1052133 and rs159153 minor alleles, the interaction between rs1052133 and smoking, were all associated with increased NPC risk.