Cornea · 2025
Comparison of 2 Commercial Media for Corneal Organ Culture and Deswelling: CorneaMax/CorneaJet Versus Tissue-C/Carry-C.
СтатьяСравнительное исследованиеНазвание и аннотация: Перевод пока не подготовлен
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Ninotta Sandrine, Toubeau David, Sagnial Tomy, He Zhiguo, Poinard Sylvain, Travers Gauthier, Gain Philippe, Thuret Gilles, Muraine Marc
Аннотация
PURPOSE: To compare the endothelial cell density (ECD) of corneas stored in organ culture (OC) using CorneaMax/CorneaJet and Tissue-C/Carry-C media.
METHODS: Three complementary experiments included paired corneas stored in CorneaMax or Tissue-C and subsequently deswelled in CorneaJet or Carry-C. In experiment 1, storage media renewal was performed only for CorneaMax as per the manufacturer's guidelines, with storage/deswelling times of 30/4 days (D). In experiment 2, both storage media were renewed on D4, with storage/deswelling time of 28/2 days. In experiment 3, both storage media were renewed, with storage/deswelling times of 22/4 days. In addition to ECD (in the three experiments), the viable ECD (Hoechst 33342/Calcein-AM staining) and central corneal thickness (CCT) were measured in exp. 2.
RESULTS: In experiment 1 (16 pairs), ECD was significantly higher in CorneaMax than in Tissue-C on D30 (2265 ± 379 vs. 2097 ± 344 cells/mm 2 , P = 0.0248) and postdeswelling (2106 ± 405 vs. 1910 ± 355 cells/mm 2 , P = 0.0290). In experiment 2 (10 pairs), ECD did not differ after 28 days of OC; however, CCT was lower on D4, higher on D28 in Tissue-C, and showed no difference after deswelling. Postdeswelling, viable ECD was significantly higher in CorneaJet versus Carry-C (1647 ± 324 vs. 1436 ± 235 cells/mm 2 , P = 0.0189). In experiment 3 (eight pairs), ECD showed no significant difference.
CONCLUSIONS: Endothelial survival is reduced in Tissue-C/Carry-C unless storage is limited to 3 weeks and the medium is renewed. The major corneal edema in Tissue-C may contribute to the reduced ECD.
METHODS: Three complementary experiments included paired corneas stored in CorneaMax or Tissue-C and subsequently deswelled in CorneaJet or Carry-C. In experiment 1, storage media renewal was performed only for CorneaMax as per the manufacturer's guidelines, with storage/deswelling times of 30/4 days (D). In experiment 2, both storage media were renewed on D4, with storage/deswelling time of 28/2 days. In experiment 3, both storage media were renewed, with storage/deswelling times of 22/4 days. In addition to ECD (in the three experiments), the viable ECD (Hoechst 33342/Calcein-AM staining) and central corneal thickness (CCT) were measured in exp. 2.
RESULTS: In experiment 1 (16 pairs), ECD was significantly higher in CorneaMax than in Tissue-C on D30 (2265 ± 379 vs. 2097 ± 344 cells/mm 2 , P = 0.0248) and postdeswelling (2106 ± 405 vs. 1910 ± 355 cells/mm 2 , P = 0.0290). In experiment 2 (10 pairs), ECD did not differ after 28 days of OC; however, CCT was lower on D4, higher on D28 in Tissue-C, and showed no difference after deswelling. Postdeswelling, viable ECD was significantly higher in CorneaJet versus Carry-C (1647 ± 324 vs. 1436 ± 235 cells/mm 2 , P = 0.0189). In experiment 3 (eight pairs), ECD showed no significant difference.
CONCLUSIONS: Endothelial survival is reduced in Tissue-C/Carry-C unless storage is limited to 3 weeks and the medium is renewed. The major corneal edema in Tissue-C may contribute to the reduced ECD.