[The expression of p27Kip1 and β-catenin in multiple endocrine neoplasia type 1-related parathyroid tumors].

Objective

To explore tissue expression of cyclin-dependent kinase inhibitor p27Kip1 and β-catenin in multiple endocrine neoplasia type1 (MEN1)-related parathyroid tumors (MHPT).

Methods

Immunohistochemistry was performed to analyze the expression of p27Kip1 and β-catenin in parathyroid glands from 31 subjects with MHPT collected at Peking Union Medical College Hospital from 2002 to 2013. Five normal parathyroid glands were used as control.

Results

In MHPT subjects, nuclear expression of p27Kip1 was absent in 4 (12.9%), weak in 10 (32.3%) and moderated staining in 17 parathyroid specimens (54.8%), respectively. While, in normal subjects, the nuclear expression of p27Kip1 was observed in all subjects and was stronger than that from MHPT subjects (P=0.001). As to the expression of β-catenin, normal parathyroid showed a distinct to moderate membrane staining, a moderate to weak cytoplasmic staining and negative nuclear staining. Similarly, MHPT exhibited a marked to moderate membrane (P=0.087), a moderated to weak cytoplasmic (P=0.357), and negative nuclear β-catenin staining.

Conclusions

The expression of p27Kip1 is reduced or absent in MHPT tissue, and no nuclear expression of β-catenin is observed in the tumors, which suggesting p27Kip1, but not β-catenin nuclear accumulation, play a role in the development of the tumors.