Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2009
[Protective effects and mechanism of N-acetylcysteine on cardiac injury induced by ischemia/reperfusion in diabetic rats].
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Zeng Shan, Lin Yi, DI Jing-fang, Feng Zheng
Аннотация
AIM: To study the protective effects and mechanism of N-acetylcysteine on cardiac injury induced by ischemia/reperfusion in diabetic rats.
METHODS: Diabetes mellitus was induced by intraperitoneal injection of streptozotocin(STZ). The animals were randomly reassigned into sham-operated group, I/R group and I/R+treated with NAC group. The content of GSH and GSSG, and the activity of Caspase-3 were measured. The apoptosis index (AI) by TUNEL staining was calculated. In addition, the apoptosis of Cardiomyocyte was also confirmed by DNA Ladder.
RESULTS: Treatment with NAC decreased the activity of Caspase-3, the content of GSSG, the values of AI but increased the content of GSH in both non-diabetic and diabetic rats (P<0.05), but there were still significant difference about the values of above parameters between diabetic rats and non-diabetic rats (P<0.05).
CONCLUSION: NAC can attenuated cardiomyocyte apoptosis by decreasing the activity of Caspase-3 and increasing the content of GSH, which has protective effect on ischemic/reperfused myocardium injury in both non-diabetic and diabetic rats, but the cardioprotective effect is less effective in diabetic rats than that in non-diabetic rats.
METHODS: Diabetes mellitus was induced by intraperitoneal injection of streptozotocin(STZ). The animals were randomly reassigned into sham-operated group, I/R group and I/R+treated with NAC group. The content of GSH and GSSG, and the activity of Caspase-3 were measured. The apoptosis index (AI) by TUNEL staining was calculated. In addition, the apoptosis of Cardiomyocyte was also confirmed by DNA Ladder.
RESULTS: Treatment with NAC decreased the activity of Caspase-3, the content of GSSG, the values of AI but increased the content of GSH in both non-diabetic and diabetic rats (P<0.05), but there were still significant difference about the values of above parameters between diabetic rats and non-diabetic rats (P<0.05).
CONCLUSION: NAC can attenuated cardiomyocyte apoptosis by decreasing the activity of Caspase-3 and increasing the content of GSH, which has protective effect on ischemic/reperfused myocardium injury in both non-diabetic and diabetic rats, but the cardioprotective effect is less effective in diabetic rats than that in non-diabetic rats.