Fetal and pediatric pathology · 2026
PANoptosis Genes as Novel Diagnostic Biomarkers for Neonatal Necrotizing Enterocolitis.
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Jia Li-Li, Luo Shu-Yue, Ye Xiao-Ya, Zhu Hong-Hong, Li Zhan-Li
Аннотация
BACKGROUND: Necrotizing enterocolitis (NEC), a life-threatening neonatal gastrointestinal disease, lacks reliable biomarkers. This study explores PANoptosis-a novel inflammatory cell death pathway-in NEC pathogenesis.
MATERIALS AND METHODS: Ileal transcriptomic profiles from surgical NEC and non-inflammatory controls were retrieved from GSE64801 and GSE46619. Data were standardized via log-transformation and quantile normalization. After identifying differentially expressed genes, we intersected them with 902 PANoptosis-related genes. A diagnostic model was developed using LASSO regression and validated via ROC, calibration, and decision curve analyses.
RESULTS: Fifty-five NEC-associated PANoptosis genes were identified, primarily enriched in inflammatory pathways. NFKBIA and PECAM1 emerged as pivotal biomarkers. The model achieved an AUC and C-index of 0.903, with calibration curves confirming reliability. DCA and clinical impact curves demonstrated significant clinical utility.
CONCLUSION: NFKBIA and PECAM1 are promising biomarkers for surgical NEC linked to PANoptosis. This study establishes a diagnostic framework and proposes therapeutic targets for advanced NEC management.
MATERIALS AND METHODS: Ileal transcriptomic profiles from surgical NEC and non-inflammatory controls were retrieved from GSE64801 and GSE46619. Data were standardized via log-transformation and quantile normalization. After identifying differentially expressed genes, we intersected them with 902 PANoptosis-related genes. A diagnostic model was developed using LASSO regression and validated via ROC, calibration, and decision curve analyses.
RESULTS: Fifty-five NEC-associated PANoptosis genes were identified, primarily enriched in inflammatory pathways. NFKBIA and PECAM1 emerged as pivotal biomarkers. The model achieved an AUC and C-index of 0.903, with calibration curves confirming reliability. DCA and clinical impact curves demonstrated significant clinical utility.
CONCLUSION: NFKBIA and PECAM1 are promising biomarkers for surgical NEC linked to PANoptosis. This study establishes a diagnostic framework and proposes therapeutic targets for advanced NEC management.