Effect of SDT on Ligature-Induced Periodontitis in Rats With and Without Diabetes.

OBJECTIVE

This study aimed to evaluate the effect of hematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on experimental periodontal disease in rats with diabetes.

METHODS

72 rats were randomly divided into two groups: non-diabetic (ND; n = 36) and diabetic (D; n = 36). Diabetes was induced by an intraperitoneal injection of streptozotocin (STZ). Periodontal disease was induced in both groups at the maxillary second molars for 4 weeks. Then the rats received the following treatments: sterile saline solution (control group); scaling and root planing (SRP group); HMME (40 μg/mL) plus 3 W/cm2 low-intensity ultrasound irradiation every other day (1 MHz, 600 s) (SRP + SDT group). All rats were euthanized at 10 days postoperatively. Half of the maxilla in each group was taken for micro-CT, and the others were given the hematoxylin and eosin and Masson's trichrome examinations.

RESULTS

Both ND and D groups demonstrated less bone loss in the SRP + SDT treatment than control and SRP treatment (p < 0.05). Results of the histometric and micro-CT were the same. In addition, histological examinations showed that less bone loss was found in the SRP treatment compared to the control treatment in the D group (p < 0.05).

CONCLUSIONS

SDT was an effective therapy for ligature-induced periodontitis in rats with diabetes.