[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 2013
[The role and mechanism of costimulatory molecule B7-H3 in the immune reaction of corneal transplantation in mice].
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Ma Ming, Wu Jing, Huang Xiao-huan, Xiong Ke, Chen Lin-jiang
Аннотация
OBJECTIVE: To investigate the role of B7-H3 in the immune reaction of corneal transplantation in mice
METHODS: Experimental study. Thirty Corneas of C57BL/6 mice were orthotopically transplanted into the eyes of BALB/c mice, and graft survival was assessed on the basis of Sonoda's standard. When the RI grade was ≥ 2, rejection was acknowledged and brought into the rejected group(R), and the others into the accepted group (A); 8 BALB/c Corneas into their own eyes belonged to isografts (I) ; In addition, 8 normal BALB/c mice were the control group (C) . At last, three eyes in each group (C, I, A, R groups) were used for HE staining and IHC of B7-H3, and there were five eyes in each group for qPCR to detect B7-H3 mRNA expression. Repeated-measures analysis of variance (factorial analysis) followed by LSD test were used for post hoc analysis for expression differences of B7-H3 mRNA between groups.
RESULTS: There were 9 accepted grafts and 21 rejected grafts in 30 mice, and transplantation survival rate was 30% in the allograft group, while all grafts were transparent, and transplantation survival rate was 100% in the isografts. IHC results showed that B7-H3 was expressed on the corneal epithelium, endothelium and iris-ciliary body of both normal corneas and Isografts; B7-H3 expression increased in the accepted group and decreased in the rejected group . The results of qPCR conformed to the IHC; Repeated-measures analysis of variance (factorial analysis) followed by LSD test were used for post hoc analysis for differences between groups (F = 429.546) . there was a low B7-H3 mRNA expression in the R group (3.89 ± 0.037) and high expression in the A group (5.04 ± 0.058); and C (4.30 ± 0.023) ,I (4.33 ± 0.031) groups had no significant difference (P = 0.387) ;But there was a significant difference between group R and C (P = 0.003)or group A and C(P = 0.001).
CONCLUSION: All above show that B7-H3 may play an important role in the maintenance of ocular immune privilege.
METHODS: Experimental study. Thirty Corneas of C57BL/6 mice were orthotopically transplanted into the eyes of BALB/c mice, and graft survival was assessed on the basis of Sonoda's standard. When the RI grade was ≥ 2, rejection was acknowledged and brought into the rejected group(R), and the others into the accepted group (A); 8 BALB/c Corneas into their own eyes belonged to isografts (I) ; In addition, 8 normal BALB/c mice were the control group (C) . At last, three eyes in each group (C, I, A, R groups) were used for HE staining and IHC of B7-H3, and there were five eyes in each group for qPCR to detect B7-H3 mRNA expression. Repeated-measures analysis of variance (factorial analysis) followed by LSD test were used for post hoc analysis for expression differences of B7-H3 mRNA between groups.
RESULTS: There were 9 accepted grafts and 21 rejected grafts in 30 mice, and transplantation survival rate was 30% in the allograft group, while all grafts were transparent, and transplantation survival rate was 100% in the isografts. IHC results showed that B7-H3 was expressed on the corneal epithelium, endothelium and iris-ciliary body of both normal corneas and Isografts; B7-H3 expression increased in the accepted group and decreased in the rejected group . The results of qPCR conformed to the IHC; Repeated-measures analysis of variance (factorial analysis) followed by LSD test were used for post hoc analysis for differences between groups (F = 429.546) . there was a low B7-H3 mRNA expression in the R group (3.89 ± 0.037) and high expression in the A group (5.04 ± 0.058); and C (4.30 ± 0.023) ,I (4.33 ± 0.031) groups had no significant difference (P = 0.387) ;But there was a significant difference between group R and C (P = 0.003)or group A and C(P = 0.001).
CONCLUSION: All above show that B7-H3 may play an important role in the maintenance of ocular immune privilege.