Changes in receptor levels for thyrotropin releasing hormone, serotonin, and substance P in cervical spinal cord of Wobbler mouse: a quantitative autoradiography study during early and late stages of the motoneuron disease.

Receptor levels for thyrotropin releasing hormone (TRH) measured by quantitative autoradiography in the Wobbler mouse cervical spinal cord show receptor losses that may relate to the inherited loss of motoneurons, most pronounced late (at Stage 4) in the motoneuron disease. An age-related decrease of TRH and serotonin (5-HT) receptors can be seen in the ventral horn of the control specimens (normal phenotype littermate and wild-type alike). However, this pattern is missing for substance P (SP) receptors from the wild-type specimens. Therefore the age-related decrease of SP receptors detected in the Wobbler mouse strain may identify a strain-related defect in SP neuronal/receptor developmental patterns. A higher level of TRH receptors was measured in the Wobbler dorsal horn at an early stage (Stage 1) in the motoneuron disease compared with the control specimens. The data are discussed in relation to an aberrant neuronal sprouting that occurs around the degenerating motoneurons in the ventral horn during the course of the motoneuron disease.