Fertility and sterility · 2003
Strategies and outcomes of the first 100 cycles of preimplantation genetic diagnosis at the Guy's and St. Thomas' Center.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Pickering Susan, Polidoropoulos Nikolaos, Caller Jenny, Scriven Paul, Ogilvie Caroline Mackie, Braude Peter, Preimplantation Genetic Diagnosis Study Group
Аннотация
OBJECTIVE: To establish strategies for the implementation of a successful preimplantation genetic diagnosis (PGD) service.
DESIGN: Retrospective review of data from a single center.
SETTING: A United Kingdom National Health Service hospital.
PATIENT(S): Patients (60 couples) were referred for PGD from UK genetic centers.
INTERVENTION(S): We followed the protocol of ovarian stimulation, oocyte retrieval, fertilization, single cell biopsy on day 3, and embryo transfer on day 4. Pregnancies unaffected by the familial genetic condition.
RESULT(S): A total of 60 couples was treated for 20 different conditions. Early cycles using nonsequential embryo culture media were less successful (13% pregnancy rate/embryo transfer) than later cycles using sequential media (33.5%). Ninety-four percent of embryos (n = 473) had a single cell removed at biopsy. The overall pregnancy rate was 24% per cycle started, 29% per egg collection, 38% per transfer, and 40% per couple treated. In one cycle, an affected pregnancy followed PGD for spinal muscular atrophy (SMA).
CONCLUSION(S): The use of sequential media and single cell biopsy results in a successful PGD program with encouraging pregnancy rates.
DESIGN: Retrospective review of data from a single center.
SETTING: A United Kingdom National Health Service hospital.
PATIENT(S): Patients (60 couples) were referred for PGD from UK genetic centers.
INTERVENTION(S): We followed the protocol of ovarian stimulation, oocyte retrieval, fertilization, single cell biopsy on day 3, and embryo transfer on day 4. Pregnancies unaffected by the familial genetic condition.
RESULT(S): A total of 60 couples was treated for 20 different conditions. Early cycles using nonsequential embryo culture media were less successful (13% pregnancy rate/embryo transfer) than later cycles using sequential media (33.5%). Ninety-four percent of embryos (n = 473) had a single cell removed at biopsy. The overall pregnancy rate was 24% per cycle started, 29% per egg collection, 38% per transfer, and 40% per couple treated. In one cycle, an affected pregnancy followed PGD for spinal muscular atrophy (SMA).
CONCLUSION(S): The use of sequential media and single cell biopsy results in a successful PGD program with encouraging pregnancy rates.
Предметные рубрики MeSH
Chromosome AberrationsCryopreservationCulture TechniquesEmbryo TransferEmbryo, MammalianFemaleFertilization in VitroGenetic Diseases, InbornHumansMaleOvulation InductionPolymerase Chain ReactionPregnancyPregnancy OutcomePreimplantation DiagnosisRetrospective StudiesSperm Injections, IntracytoplasmicTreatment OutcomeUnited Kingdom