Zhen ci yan jiu = Acupuncture research · 2011
[Effect of acupuncture on gastrointestinal stem cell factor/kit system after colocolic anastomosis in rats].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Deng Jing-Jing, Liu Xiao-Rong, Yuan Qing
Аннотация
OBJECTIVE: To observe the effect of acupuncture on colonic stem cell factor (SCF)-receptor tyrosine kinase (Kit, a transmembrane protein c-kit) system in colocolic anastomosis rats so as to explore its underlying mechanism in regulating intestinal motility after enteroenterostomy.
METHODS: Thirty SD rats were randomized into control, model (colocolic anastomosis) and acupuncture groups (n = 10/group). Acupuncture was applied to bilateral "Zusanli" (ST 36), "Sanyinjiao" (SP 6) and "Taichong" (LR 3), once a day for 3 days. The fecal-excretion time and the propulsive rate of the small intestine were measured. The immunoactivity of c-kit immunoreaction positive products in the colon tissue 2 cm below the caecum was detected using immunohistochemistry and the expression of SCF mRNA detected using real time-polymerase chain reaction (RT-PCR) method.
RESULTS: Following colocolic anastomosis, the intestinal propulsive rate in the model group was decreased considerably in comparison with the control group (P < 0.01). Compared with the model group, the first fecal-excretion time and the intestinal propulsive rate were improved significantly in the acupuncture group (P < 0.05). In comparison with the control group, the immunoactivity of c-kit and the expression of SCF mRNA in colon tissue were down-regulated obviously in the model group (P < 0.05, P < 0.01), while in comparison with the model group, the expression levels of c-kit and SCF mRNA were increased in the acupuncture group significantly (P < 0.05).
CONCLUSION: Acupuncture can suppress enteroenterostomy-induced down-regulation of the expression of c-kit protein and SCF mRNA in colocolic anastomosis rats, which may contribute to its effect in regulating the intestinal motility after enteroenterostomy.
METHODS: Thirty SD rats were randomized into control, model (colocolic anastomosis) and acupuncture groups (n = 10/group). Acupuncture was applied to bilateral "Zusanli" (ST 36), "Sanyinjiao" (SP 6) and "Taichong" (LR 3), once a day for 3 days. The fecal-excretion time and the propulsive rate of the small intestine were measured. The immunoactivity of c-kit immunoreaction positive products in the colon tissue 2 cm below the caecum was detected using immunohistochemistry and the expression of SCF mRNA detected using real time-polymerase chain reaction (RT-PCR) method.
RESULTS: Following colocolic anastomosis, the intestinal propulsive rate in the model group was decreased considerably in comparison with the control group (P < 0.01). Compared with the model group, the first fecal-excretion time and the intestinal propulsive rate were improved significantly in the acupuncture group (P < 0.05). In comparison with the control group, the immunoactivity of c-kit and the expression of SCF mRNA in colon tissue were down-regulated obviously in the model group (P < 0.05, P < 0.01), while in comparison with the model group, the expression levels of c-kit and SCF mRNA were increased in the acupuncture group significantly (P < 0.05).
CONCLUSION: Acupuncture can suppress enteroenterostomy-induced down-regulation of the expression of c-kit protein and SCF mRNA in colocolic anastomosis rats, which may contribute to its effect in regulating the intestinal motility after enteroenterostomy.